Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When…

Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The…

β-NADH, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. β-NAD+ is a carrier for hydride ion, forming β-NADH. The hydride ion is enzymatically removed from a substrate molecule by the action…

Heparin is a polymer classified as a mucopolysaccharide or a glycosoaminoglycan. It is biosynthesized and stored in mast cells of various mammalian tissues, particularly liver, lung and mucosa. It is made up of repeated disaccharide units consisting of 1,4-linked L-iduronic acid and D-glucosamine.…

Aprotinin is a single chain polypeptide (58 amino-acids) crosslinked by three disulfide bridges, showing competitive and reversible inhibiton of proteolytic and esterolytic activity. Aprotinin forms stable complexes with, and blocks the active sites of serine protease enzymes. It is found in bovine…

Linoleic acid (LA) is an unsaturated n-6 fatty acid; it belongs to one of the two families of essential fatty acids. The body cannot synthesize linoleic acid from other food components. Linoleic acid can be used to show the antioxidant effect of natural phenols.

Dexamethasone is an anti-inflammatory glucocorticoid that induces the production of phospholipase A2 inhibitory protein (lipocortin). It also inhibits induction of nitric oxide synthase (IC50=5 nM). Dexamethasone has been shown to cause reduction in cyclin A and Cdk2 activity, inhibition of G1/S…

Cefsulodin is a third generation cephalosporin antibiotic that has very specific activity against Pseudomonas aeruginosa. It has no significant activity against other Gram-negative bacteria and very limited activity against Gram-positive bacteria and anaerobic bacteria. Cefuslodin is most…

Apoferritin is the Protein shell of ferritin. Calibration of gel filtration columns and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis Acts as an anti-oxidant When added to cultured endothelial cells apoferritin is taken up in a dose-responsive manner and appears to…

β-NAD is one of the biologically active forms of nicotinic acid. Occurs in living cells, primarily in the oxidized state Coenzyme for dehydrogenases NAD usually acts as a hydrogen acceptor, forming NADH Major electron acceptor molecule in biological oxidations. Acts as a…

Collagenases degrade native helical collagen fibrils. Plays an important role in connective tissue metabolism Produced by specific cells involved in repairs and remodeling processes Type II enzyme that contains greater clostripain activity Collagenase is used for collagen…

Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. Molecular weight has been determined by Viscosity as well as by HPLC. It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction…

Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolized in the tricarboxylic acid cycle to produce…

Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase from Clostridium histolyticum has extensive use in biological studies, where it is used to…

Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents. The agglutination activity of these highly specific carbohydrate-binding molecules is…

Poly-L-lysine is a positively charged amino acid polymer. Poly-lysine binds to DNA, red cell membrane and any negatively charged protein. It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell…

Guanosine-5'-triphosphate, a purine nucleotide, is required as a coenzyme for protein biosynthesis in a cell-free system. Guanosine 5′-triphosphate (GTP), a purine trinucleotide, is a substrate for RNA polymerases and a wide range of GTPase including G-protein coupled receptor (GPCR)…

D-Biotin is a growth factor present in small amounts in every living cell. Involved in carboxylation reactions Occurs mainly bound to proteins or polypeptides Abundant in the liver, kideny, pancreas, yeast, and milk Levels are higher in cancerous tumors than in normal tissues …

Collagenases degrade native helical collagen fibrils. It has an important role in connective tissue metabolism and is produced by specific cells involved in repairs and remodelling processes. Collagenase is used for tissue dissociation combined with other enzymes such as elastase, trypsin,…

Biliverdin Dihydrochloride is a bile pigment component. An oxidized metabolite of bilirubin caused when bilirubin reacts with reactive oxygen species. Biliverdin IX is composed of alpha, beta, gamma and delta isomers. Biliverdin dihydrochloride was found responsible for cytoprotection. It was…

Poly-L-lysine is a positively charged amino acid polymer. Poly-lysine binds to DNA, red cell membrane and any negatively charged protein. It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell…

Tamoxifen is a Protein Kinase C inhibitor. It induces apoptosis in human malignant glioma cell lines. Tamoxifen and its metabolite 4-hydroxytamoxifen are selective estrogen response modifiers (SERMs) that act as estrogen antagonists in mammary gland. Blocks estradiol-stimulated VEGF production in…

Suitable for cell adhesion in tissue culture, as well as gel formation, platelet aggregation, and as a substrate for collagenase. Collagen is a group of naturally occurring proteins found in animals, especially in the flesh and connective tissues of vertebrates. It is the main component of…

Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding. A compromise between the easier to use lower molecular weight products…

Albumins are a group of simple proteins found in the body fluids and tissues of animals and in some plant seeds. Unlike globulins, albumins have low molecular weights, are soluble in water, are easily crystallized and contain an excess of acidic amino acids. Serum and plasma albumin is…

Elastase is an enzyme from the class of proteases (peptidases) that break down proteins. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids. Hydrolyses elastin Digests hemoglobin, casein, and fibrin One unit will solublize 1 mg of elastin in 20 minutes at…

N-Acetyl-D-galactosamine(GalNAc), an aminosugar, is a component of many O-linked and N-linked glycan structures. Intial O-linked sugar to many serine and threonine residues in protein glycosylations.

Papain is a sulfhydryl protease from Carica papaya latex. Cleaves peptide bonds of basic amino acids, leucine, or glycine Hydrolyzes esters and amides One unit will hydrolyze 1.0 µmole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25°C and pH 6.2 Papain…

β-NADP is a coenzyme necessary for the alcoholic fermentation of glucose and the oxidative dehydrogenation of other substances. It occurs widely in living tissue, especially in the liver. Nicotinic acid can be converted to nicotinamide in the body and, in this form, is found as a component of…

Human chorionic gonadotropin (HCG), a polypeptide hormone produced by the human placenta, is composed of an alpha and a beta sub-unit. The alpha sub-unit is essentially identical to the alpha sub-units of the human pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone…

Hyaluronidase is a glycoprotein containing 5% mannose and 2.17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- β-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a…

Fetuin is a protein containing 12 Cys residues, all involved in disulfide bridges. The composition of bovine fetuin (weight %) is polypeptide 74%, hexose 8.3%, hexosamines 5.5%, and sialic acid 8.7%. Fetuin bovine is a protein with the ability to bind to the insulin receptor and inhibit insulin…