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MP Biomedicals

Poly-L-Lysine, MW >=70,000

100 out of 100

Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding.

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor. Poly-L-Lysine is typically used as a coating substrate for culture dishes, slides, etc. It can be used in conjugation to methotrexate for increased drug transport. For Microencapsulation of islets. It is also use in simple reproducible procedure for chromosomal preparations from a variety of tissues. For immobilation of living cells in biocompatible semipermeable microcapsules. In the preparation of polycationic beads. Conjugation to albumin and horseradish peroxidase to enhance cellular uptake. Poly-lysine with mol. wt. >70,000 is useful in promoting cell adhesion to solid substrates.

All molecular weights are calculated from the viscosity of the parent compound Poly-epsilon-Z-L-Lysine.

Stock Solution for coating of culture dishes/slides: Dissolve 10 mg poly-L-lysine in 1 mL sterile, deionized water. This creates a 1% solution. Effective coating solution concentration for culture dishes will be 0.3 to 0.5% and for histology slides at 0.1%.

Key Applications: Coating substrate for culture dishes, slides. Cell adhesion. Vector for gene therapy

Product Type: Biochemicals

Presentation: Cream Powder

Format: Powder

Molecular Weight: ≥70,000

Solubility: Soluble in water (50mg/mL).

Storage & Handling: -20°C, desiccate.

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Product Detail
Thomas No.
C829P20
Mfr. No.
0215017710
CAS #
25988-63-0
Description
Poly-L-Lysine, MW >=70,000, 10mg
Packaging Size
10 mg
Packaging Type
Bottle, Glass, Amber
list price/quantitytotal
$0.00
Thomas No.
C829P21
Mfr. No.
0215017725
CAS #
25988-63-0
Description
Poly-L-Lysine, MW >=70,000, 25mg
Packaging Size
25 mg
Packaging Type
Bottle, Glass, Amber
list price/quantitytotal
$0.00
Thomas No.
C829P22
Mfr. No.
0215017750
CAS #
25988-63-0
Description
Poly-L-Lysine, MW >=70,000, 50mg
Packaging Size
50 mg
Packaging Type
Bottle, Glass, Amber
list price/quantitytotal
$0.00
Thomas No.
C829P23
Mfr. No.
0215017780
CAS #
25988-63-0
Description
Poly-L-Lysine, MW >=70,000, 100mg
Packaging Size
100 mg
Packaging Type
Bottle, Glass, Amber
list price/quantitytotal
$0.00
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Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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  • Reviewed on Tuesday, August 18, 2020

    100 out of 100

    Comments
    good for coad the plate