Bsu DNA Polymerase I, Large Fragment is a product of the Bacillus subtilis DNA polymerase I which lacks the N-terminal exonuclease domain (1-296 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity.…

T4 gp32 Protein is a single-stranded DNA binding protein required for T4 DNA replication, recombination, and repair (1, 2).  It improves the efficiency of reverse transcriptase (RT) during RT-PCR (3), enhances T4 DNA polymerase activity (4), as well as increases the yield of PCR products (5). …

Intact Genomics Taq DNA Polymerase 2x Premix with Dye is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.…

Intact Genomics T4 DNA Polymerase has both a DNA-dependent DNA polymerase activity and a potent 3´→5´ exonuclease activity.   Product Includes: T4 DNA Polymerase 10x T4 DNA Polymerase Buffer

igScript™ RT-PCR Kit combines two powerful mixtures: i). 5x igScript™ master mix and ii) Hot start Taq 2x master mix with standard buffer for 2-steps RT-PCR. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.c Product…

i7™ High-Fidelity DNA Polymerase 2x master mix is a ready to use premix which contains high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer.  i7™ High-Fidelity DNA Polymerase 2x master mix has the high-fidelity,…

Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ 10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat…

Intact Genomics chemically competent DL39 (DE3) E. coli cells are specific for transformation and protein expression in order to uniformly and specifically label :e.g. phenylalanine or leucine residues.  DL39 (DE3) can also be used to reduce NMR cross-labeling via transaminase activity for…

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids (1). The CRISPR system consists of a…

Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ TG1 Phage Display chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by…

IgScript™ Probe-Based One Step RT-qPCR 2x master mix contains IgScript™ Reverse Transcriptase, Taq DNA polymerase, MgCl2, dNTPs, stabilizers and low ROX reference dye with standard buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity.…

T5 Exonuclease is a highly efficient 5´→3´ exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions. However, the enzyme does not degrade supercoiled dsDNA (1). The mode of action of T5 Exonuclease in vivo may be…

Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps…

i7™ High-Fidelity DNA Polymerase has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes. Applications: Long and difficult template DNA…

T7 Endonuclease I, a stable homodimer of identical 149 amino acid subunits (1) is the product of a recombinant gene in E. coli . Double-stranded breaks (DSBs) generated by CRISPR/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form…

Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA.  This enzymes joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex…

Intact Genomics FastAmpTM Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need of complicated DNA purification steps.  The advanced formulation of this kit also allows fast PCR cycling…

i7™ Hot Start High-Fidelity DNA Polymerase has the high-fidelity, sensitivity and processivity with an error rate ~2.8×10^2 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes. Applications: Long and difficult template DNA…

Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression. Product Includes: BL21 (DE3) competent cells pUC19 control DNA Recovery medium

Intact Genomics 5-alpha electrocompetent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning. Mutations in endA1 and recA1 ensure increased plasmid yield and improved plasmid quality. …

Intact Genomics ig™ 10B (DH10B derivative) electrocompetent E. coli cells offer the highest transformation efficiencies of ≥5 x 1010 cfu/µg plasmid DNA which are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. …

Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease…

The AGL1  strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542…

Intact Genomics EHA105 ElectroComp Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice,…