T5 Exonuclease is a highly efficient 5´→3´ exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions. However, the enzyme does not degrade supercoiled dsDNA (1). The mode of action of T5 Exonuclease in vivo may be analogous to that of the 5´→3´ exonuclease activity of E. coli DNA Polymerase I
10x T5 Exonuclease Reaction Buffer
•Useful for Plasmid mutagenesis methods, oligonucleotide site-directed mutagenesis, generation of plasmid-sequencing templates, generation of 3’-overhang for improved cloning procedures.
• Extremely high purity.
• Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product.
• The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining.