The GenBuilder Cloning Kit enables seamless and one-step cloning of multiple DNA fragments into a linearized vector. The proprietary enzyme mix fuses together DNA fragments efficiently and precisely by recognizing 15-40 bp overlaps at the end of each fragment. The system allows cloning of DNA…

P2Y4 is a receptor that is activated by UTP specifically. Activation of P2Y4 results in increased inositol phosphate formation and mobilization of intracellular calcium. Although selective antagonists for P2Y4 are still lack, P2Y4 may play an important role in vascular diseases, and…

5X Sample Buffer is used as a tracking dye for SDS PAGE gel loading. This buffer contains SDS and is suitable for denaturing gel electrophoresis. Dilute 1:4 with sample before loading. Note: 5X Sample Buffer should be brought to room temperature prior to use. Mix well before loading gel.

Adeno-associated virus type 8 (AAV8) is a valuable vector for gene therapy. It is one member of the human parvovirus family which depends on co-infection with helper viruses such as adenovirus or herpes virus for efficient reproduction. AAV8 empty capsids are composed of three proteins, VP1, VP2…

Muscarinic acetylcholine receptors belong to a superfamily of seven-™-domain receptors that interact with G-proteins to initiate intracellular responses. Five muscarinic receptor subtypes have been identified, named M1 through M5. Receptors of the M5 receptor subtype couple through the…

Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The SNAP-tag is a self-labeling protein tag that forms a highly stable, covalent thioether bond with fluorophores and other substituted groups on its benzyl guanine substrate.…

Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The production of EPO by kidney cells is increased in response to hypoxia or anemia.…

The alpha factor pheromone arrests yeast in the G1 phase of their cell cycle. Alpha Factor Mating Pheromone induces the expression of mating genes, changes in nuclear architecture, and polarzes growth toward the mating partner. STE2 encodes the alpha factor pheromone receptor (a GPCR) found on…

SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), also known as 2019-nCoV, is a positive-sense single-stranded RNA virus. It caused coronavirus disease 2019 (COVID-19). Nucleocapsid Protein is a most abundant structure protein of the coronavirus which is associated with nucleic acid. …

Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used in PCR. In some cases, such as RAPD PCR, adding large volume of general Taq DNA polymerase (5 U/µl), which…

CD155, commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of…

Lysophosphatidic acid (LPA), a bioactive lipid produced by several cell types including pos™itotic neurons and activated platelets, is thought to be involved in various biological processes, including brain development. Three cognate G-protein coupled receptors encoded by…

Angiotensin receptor 1 (AT1 receptor) is a member of angiotensin receptors which are responsible for the signal transduction of the main effecter hormone. Effects mediated by the AT1 receptor include vasoconstriction, aldosterone synthesis and secretion, increased vasopressin secretion, cardiac…

Dupilumab with trade name Dupixent, is an FDA-approved drug for the treatment of patients aged 6 years and older with moderate-to-severe atopic dermatitis. It is a human monoclonal antibody. Dupilumab binds to the interleukin-4 receptor alpha (IL-4Rα) subunit shared by the interleukin (IL)-4…

B-cell activating factor, also known as BAFF, TALL-1, TNAK, and zTNF4, is a member of theTNF ligand superfamily designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation. BAFF binds to three TNF…

10*10 buffer dam for GenBox Mini Electrophoresis Tank. One-piece buffer dam is used when running only one gel.

Neuregulin is a signaling protein for ErbB2/ErbB4 receptor heterodimers on the cardiac muscle cells, playing an important role in heart structure and function through inducing ErbB2/ErbB4 receptor phosphorylation and cardiomyocyte differentiation. Research on molecular level discovered that…

Pembrolizumab (Keytruda) is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of patients with unresectable or metastatic melanoma. Pembrolizumab binds to the human cell surface receptor PD-1 (Programmed Cell Death Protein 1) and blocks its…

GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.

Protein A affinity chromatography resin is the most commonly applied method for the isolation and purification of IgG. Protein A is a cell wall component found in several strains of Staphylococcus aureus . It has five high affinity binding sites capable of binding specifically to the Fc region of…

Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria . It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report…

Interleukin-6 (IL-6), also known as BSF-2, CDF and IFNB2, is a pleiotropic cytokine that acts in both pro-inflammatory and anti-inflammatory responses. It is produced mainly by T cells, macrophages, monocytes, endothelial cells and muscle cells. IL-6 binds to IL-6 receptor (IL-6R) to trigger the…

BDCA-2 is a novel type II C-type lectin, which shows 50.7% sequence identity at the amino acid level to its putative murine ortholog, the murine dendritic cell–associated C-type lectin 2. In addition to its antigen capturing function, BDCA-2 can mediate potent inhibition of induction of IFN-a/ß…

VEGF-A121 is one of five isoforms (121, 145, 165, 189, and 206) of VEGF protein, a cytokine belonging to the Platelet Differentiation Growth Factor (PDGF) family, and existing as a disulfide-linked homodimeric glycoprotein. In contrast to the longer isoforms, VEGF-A121 is more freely diffusible,…

Glucagon regulates blood glucose via control of hepatic glycogenolysis and gluconeogenesis and via regulation of insulin release from the ß cell. Pharmacological administration of glucagon increases blood glucose in normal and diabetic subjects, and produces positive inotropic and chronotropic…

Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP tagged protein is purified with an…

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. ICOS (Inducible T-cell COStimulator) is expressed on activated T cells. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. It is…

Macrophage Colony Stimulating Factor (M-CSF) ,also known as CSF1,is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. The active form of the protein is found extracellularly as a…

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni 2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni 2+ …

The Benz-Neburase is a genetically engineered endonuclease from Serratia marcescens used as a DNA eraser in the purification processes of biological molecules. The enzyme cleaves all forms of DNA and RNA into smaller nucleotides of around 5-8 base pairs. Benz-Neburase requires divalent cation,…

Broad Multi Color Pre-Stained Protein Standard is a special-designed protein standard. The standard is a mixture of ten highly purified prestained proteins ranging from 5 kDa to 270 kDa, covalently coupled with four different chromophores. The 30 kDa band is red, 50 kDa band is green, 270 kDa band…

Stem Cell Factor (SCF) is a hematopoietic growth factor that binds to the c-Kit receptor. SCF exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act…