The GenBuilder Cloning Kit enables seamless and one-step cloning of multiple DNA fragments into a linearized vector. The proprietary enzyme mix fuses together DNA fragments efficiently and precisely by recognizing 15-40 bp overlaps at the end of each fragment. The system allows cloning of DNA fragments into any linearized vector, requires no pre-existing recombination sites or helper sequences, and eliminates the need for a complicated restriction and ligation process. The GenBuilder Cloning kit is recommended for simultaneous cloning of up to five DNA fragments. For a greater number of fragments, or for assembling ssDNA oligos with dsDNA fragments, the GenBuilder Plus Cloning kit (L00744) is recommended.