Tricine, a zwitterionic buffer, was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. The name tricine comes from tris and glycine from which it was derived. Tricine can be used in…

Ammonium persulfate is a strong oxidizing agent. It is a radical initiator. Ammonium persulfate is a polymerization catalyst used with TEMED for polyacrylamide gel formation. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry. Other…

MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. Concentrations higher than 20 mM should not be used with mammalian cell cultures.

Cholesterol is the principal sterol of the higher animals, found in all body tissues. It is the main constituent of gallstones and all biological membranes. Approximately 25% of total brain lipid is cholesterol. Cholesterol is suitable for use in liposome preparations.

Mannose is a sugar monomer of the aldohexose series of carbohydrates. Mannose is a C-2 epimer of glucose. Mannose is important in human metabolism, especially in the glycosylation of certain proteins. Several congenital disorders of glycosylation are associated with mutations in enzymes involved in…

PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue samples. This will help prevent the loss of lipids. It is also used to buffer glutaraldehyde and formaldehyde when…

MOPS is used as a biological buffer. MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES; however, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4. A buffer using MOPS sodium salt can be…

Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae. Allows recovery of active DNA after separation, with minimal inhibition of restriction and ligation enzymes Suitable for both analytical and preparative electrophoresis procedures Good…

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the…

L-arginine is an amino acid. Necessary for the production of protein Helps rid the body of ammonia Stimulates the release of insulin Used to make nitric oxide Substrate of nitric oxide synthase, which is converted to citrulline and nitric oxide (NO). Induces insulin release by a…

AlbumiNZ™ Low Fatty Acid BSA supports growth of stem cells and increases protein production from hybridoma cells. AlbumiNZ™ Low Fatty Acid BSA is chromatographically purified from New Zealand-sourced bovine plasma in an ISO quality system assuring complete traceability and…

β-D-Glucose is critical for production of proteins and the lipid metabolism. β-D-Glucose is a main source of energy for living organisms and is combined into glucosides, disaccharides, oligosaccharides, polysaccharides (cellulose and starch), and glycogen. D-Glucose, a naturally…

Saponin is "built" from quillaic acid, a sugar and possibly other substances. It is a Glycoside widely distributed in plants. 1% of saponin solution can hemolyze erythrocytes. Saponin solutions have been used in the following ways: concentrations of 0.005%-0.01% were used to…

Boric acid has a single boron; its salt forms complex equilibria in solution, with the ion B4O7-2 being the predominant species. Boric acid is used for weatherproofing wood and fireproofing fabrics; as a preservative; manufacturing of cements, crockery, porcelain, enamels, glass, borates,…

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

A vitamin/enzyme cofactor found in milk, eggs, malted barley, liver, kidney, heart, leafy vegetables. Richest natural source is yeast. Occurs in the free form only in the retina of the eye, in whey, and in urine; bioactive forms occurring in tissues and cells are riboflavin monophosphate and…

Cesium chloride , an inorganic compound, is an important source of cesium ions in a variety of applications. Its crystal structure forms a major structural type where each cesium ion is coordinated by 8 chlorine ions. Cesium chloride occurs naturally in mineral waters and as an impurity in…

Benzalkonium Chloride is a quaternary ammonium salt. It is a cationic surface active agent and germicide. It is not effective against gram-negative bacteria or picornaviruses and it is not recommended for treatment of sanitizing critical or semicritical medical instruments. It has been reported…

Albumins are a group of acidic proteins which occur in the body fluids and tissues of mammals and in some plant seeds. Serum and plasma albumin is carbohydrate-free and comprises 55-62% of the protein present. However, only about 40% of the total albumin in the body is in the circulating plasma at…

L-Asparagine is used in cell culture media and is a component of MEM non-essential amino acids solution. L-Asparagine has been shown to enhance ornithine decarboxylase activity in cultured human colon adenocarcinoma Caco-2 cells and in cultured IEC-6 intestinal epithelial cells. Spore germination…

Bovine albumin is a single polypeptide chain consisting of approximately 583 to 595 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group. Albumin binds water, Ca2+, Na+, and K+. Due to a hydrophobic cleft, albumin binds fatty…

Dextran sulfate sodium salt, or DSS, is a synthetic sulfated branched polysaccharide derivative of dextran that has multiple uses in biomedical and clinical research. One important property of DSS is to trigger colitis in mice and rats when administered in drinking water. DSS binds to…

Lecithin is a purified, wax-like phosphatidylcholine of soybean origin. In addition to being the major structural component of cellular membranes, phosphatidylcholine serves as a reservoir for several lipid messengers, it is the source of the bioactive lipids lysophosphatidylcholine, phosphatidic…

* Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins * Used in the isolation of RNA * Solubilizes insoluble or denatured proteins such as inclusion bodies * Can be used for the recovery of periplasmic proteins

Deoxycholic Acid Sodium Salt Monohydrate is an anionic biological detergent for the solubilization of membrane-bound proteins. Anionic detergent; useful for extraction of membrane receptors and other plasma membrane proteins and for nuclei isolation. Deoxycholic acid has been used in a modified…

Albumins are a group of acidic proteins which occur in the body fluids and tissues of mammals and in some plant seeds. Serum and plasma albumin is carbohydrate free and comprises 55-62% of the protein present. Bovine albumin is a single polypeptide chain consisting of approximately 583 to 595 amino…

Cholesterol is the principal sterol of the higher animals, found in all body tissues. It is the main constituent of gallstones and all biological membranes. Approximately 25% of total brain lipid is cholesterol. Cholesterol, followed by the chemical suffix -ol for an alcohol, is an organic chemical…

β-D-Glucose is a main source of energy for living organisms. It is combined into glucosides, disaccharides, oligosaccharides, the polysaccharides (cellulose and starch), and glycogen. β-D-glucose may contain up to 10% of α-isomer.

Agarose is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marinealgae. As a gelling agent, agarose is used: 1.) to separate nucleic acids electrophoretically because…

L-Cysteine hydrochloride monohydrate (LCHCMH) is a water soluble salt of the non-essential amino acid, L-cysteine. Widely employed in the medicine industry Thermodynamic features of a solution of LCHCMH in water have been reported NMDA glutamatergic receptor antagonist L-Cysteine…

L-histidine is an essential amino acid with a basic side chain, and is hydrophilic in character. It contains an imidazole group in the side chain. Histidine is biosynthesized from ATP, 5-phosphoribosyl-1-pyrophosphate (PRPP), and glutamine. In turn, histidine is degraded to glutamate by histidase,…

L-(+)-Arabinose is the naturally occurring isomer and is a constituent of plant polysaccharides. L-(+)-Arabinose is used as a substrate to identify, differentiate and characterize pentose sugar isomerase(s). L-Arabinose is used in the bioproduction of L-ribose. L-Arabinose is the naturally…