Cayman Chemical, 96 Well, Chemicals
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COX-2 (human) Inhibitor Screening Assay Kit
Cayman ChemicalCOX-1 and COX-2 catalyze the production of the PG and TX precursor PGH2. COX-2 is primarily responsible for the biosynthesis of PGs under acute inflammatory conditions. The COX-2 (human recombinant) Inhibitor Screening Assay measures PGF2α that is produced by SnCl2 reduction of COX-2-derived…
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Protein Synthesis Assay Kit
Cayman ChemicalCayman’s Protein Synthesis Assay Kit is a non-radioactive method for measuring protein synthesis that employs the cell-permeable, alkyne-containing, puromycin analog O-Propargyl-puromycin (OPP). Upon application to cells, the OPP probe incorporates into the C-terminus of translating…
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ß-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit
Cayman Chemicalβ-Hydroxybutyrate (β-HB; 3-hydroxybutyric acid) is produced in the liver, mainly from the oxidation of fatty acids, and exported to peripheral tissues for use as an energy source. Measurement of β-HB can provide a reliable index of the level of normal ketosis as well as detect…
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Glycerol Colorimetric Assay Kit
Cayman ChemicalGlycerol is the backbone of triglycerides and an important intermediate in energy metabolism being involved in both oxidative and synthetic processes. The circulating levels of glycerol and free fatty acids are considered to reflect lipolysis and are therefore useful parameters to evaluate in…
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Aldosterone ELISA Kit
Cayman ChemicalAldosterone, a steroid hormone secreted by the adrenal cortex, is the principle mineralocorticoid controlling sodium and potassium balance. Serum aldosterone levels in normal upright adult individuals are generally less than 300 pg/ml. Only a fraction of urinary aldosterone is excreted intact, thus…
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Nitrate/Nitrite Colorimetric Assay Kit (LDH method)
Cayman ChemicalNADPH is an essential cofactor for the function of the NOS enzyme. Unfortunately, NADPH interferes with the chemistry of the Griess reagents, which are the most commonly used reagents for nitrite detection. This kit uses Lactate Dehydrogenase (LDH) to oxidize the excess NADPH used in a…
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COX (ovine/human) Inhibitor Screening Assay Kit
Cayman ChemicalThe COX (ovine/human) Inhibitor Screening Assay directly measures PGF2α produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via ELISA using a broadly specific antibody that binds to all the major PG compounds. Thus, the Cayman COX Assay is more accurate and…
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RARy (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor gamma (NR1B3), a ligand-dependent transcription factor commonly referred to as RARG or RARγ. INDIGO’s reporter cells…
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Anti-Citrullinated Human Fibrinogen Assay Kit (mouse)
Cayman ChemicalImmunization of mice with citrullinated human fibrinogen, especially mice expressing the human HLA-DR4 transgene, induces an arthritic response driven by the production of antibodies that recognize citrullinated epitopes. The polyclonal antibody response produces antibodies reactive with both…
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Glutathione Peroxidase Assay Kit
Cayman ChemicalGPx catalyzes the reduction of hydroperoxides, including H2O2, using reduced glutathione and thereby functions to protect the cell from oxidative damage. Cayman’s Glutathione Peroxidase Assay measures GPx activity indirectly by a coupled reaction with GR. Oxidized GSSG, produced upon…
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Mouse IgG ELISA Kit
Cayman ChemicalImmunoglobulin IgG provides the majority of antibody-based immunity against invading pathogens and plays a significant role in the pathology of auto-immune diseases. Most mouse monoclonal antibodies are of the IgG isotype. Because mice are widely used as a research model for studies of the immune…
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Triglyceride Colorimetric Assay Kit
Cayman ChemicalThe measurement of TG levels, in conjunction with other lipid assays, are useful in the diagnosis of primary and secondary hyperlipoproteinemia, dyslipidemia, and triglyceridemia. Cayman’s TG Assay provides a simple, reproducible, and sensitive tool for assaying TGs in plasma, serum, cell…
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Glutathione Assay Kit
Cayman ChemicalCayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The kit can also be used to measure only GSSG by following an alternative…
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TR (human) Reporter Assays Panel
Cayman ChemicalINDIGO’s panel of TR reporter assays utilizes non-human mammalian cells engineered to express human thyroid hormone receptors alpha (NR1A1) and beta (NR1A2), ligand-dependent transcription factors commonly referred to as TRα and TRβ. INDIGO’s TR reporter cells include the…
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TBARS Assay Kit
Cayman ChemicalOxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from polyunsaturated fatty acids results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically…
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RARß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor, beta (NR1B2), a ligand-dependent transcription factor commonly referred to as RARB or RARβ. INDIGO’s reporter cells…
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Catalase Assay Kit
Cayman ChemicalCatalase is an ubiquitous antioxidant enzyme that is present in most aerobic cells. It is involved in the detoxification of H2O2, a ROS that is a toxic product of both normal aerobic metabolism and pathogenic ROS production. Cayman’s Catalase Assay utilizes the peroxidatic function of…
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Anti-Ovalbumin IgE (mouse) ELISA Kit
Cayman ChemicalImmunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response. OVA/alum immunized mice produce anti-OVA antibodies predominantly of the IgG1 and IgE isotypes that…
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11-keto Testosterone ELISA Kit
Cayman Chemical11-KT is a second key androgenic steroid found in fish. It occurs in males together with testosterone in amounts which vary from less than 1 ng/ml to as much as 50-100 ng/ml, depending on the species and the stage of the reproductive cycle.
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LXRß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human liver X receptor beta (NR1H2), a ligand-dependent transcription factor commonly referred to as LXRβ. INDIGO’s reporter cells include the…
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ERß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the full-length human estrogen receptor 2 (NR3A2), a ligand-dependent transcription factor commonly referred to as ERβ. INDIGO’s reporter cells include the…
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RXRß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoid X receptor beta (NR2B2), a ligand-dependent transcription factor commonly referred to as RXRB or RXRβ. INDIGO’s reporter cells include…
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Prostaglandin D2 ELISA Kit
Cayman ChemicalProstaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kDa glutathione-independent lipocalin-type PGD2 synthase. This PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a…
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Oxytocin ELISA Kit
Cayman ChemicalOxytocin is a hypothalamic peptide hormone that is stored in the posterior pituitary gland. It is released into the bloodstream during parturition and lactation and is also involved in other social and sexual behaviors, neuropsyciatric disorders, and the maintenance of water and sodium homeostasis.…
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Leukotriene C4 ELISA Kit
Cayman ChemicalLeukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid in eosinophils, mast cells, and macrophages. Their biosynthesis proceeds via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The…
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sPLA2 (Type V) Inhibitor Screening Assay Kit
Cayman ChemicalThe sPLA2 (Type V) Inhibitor Screening Assay is a convenient colorimetric assay designed for rapid screening of Type V sPLA2 inhibitors in a 96-well format. Each kit contains assay buffer, DTNB, diheptanoyl thio-PC, human sPLA2 (Type V), a 96-well plate, plate cover, and complete instructions.
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Prostaglandin E2 ELISA Kit - Monoclonal
Cayman ChemicalCayman’s PGE2 ELISA is a sensitive competitive assay that uses a high-affinity PGE2 monoclonal antibody for quantification of PGE2 in plasma, urine, and culture media samples.
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Oxygen Consumption Rate Assay Kit (MitoXpress® Xtra HS Method)
Cayman ChemicalThe oxygen consumption rate (OCR) of cells is an important indicator of normal cellular function. It is used as a parameter to study mitochondrial function as well as a marker of factors triggering the switch from healthy oxidative phosphorylation to aerobic glycolysis in cancer cells. Oxygen…
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Cyclic GMP ELISA Kit (without Acetic Anhydride)
Cayman ChemicalcGMP, synthesized from GTP by both membrane-bound and soluble guanlyate cyclase enzymes, is a key intracellular second messenger molecule that transduces cellular signaling events in response to a variety of hormones, autacoids and drugs. The relative abundance of cGMP within a given cell can serve…
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ß-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit
Cayman Chemicalβ-HB is a "ketone body" which is produced in the liver, mainly from the oxidation of fatty acids, and is exported to peripheral tissues for use as an energy source. Normally ketosis can indicate that lipid metabolism has been activated and the pathway of lipid degradation is intact.…
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11ß-Prostaglandin F2a ELISA Kit
Cayman Chemical11β-PGF2α is the primary plasma metabolite of PGD2 in vivo, the levels of which can increase from 6 pg/ml in a normal healthy volunteer to 490 ng/ml in patients with systemic mastocytosis. The normal human urinary excretion of 11β-PGF2α is about 11 ng/mmol creatinine (~400…
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MitoCheck® Complex II Activity Assay Kit
Cayman ChemicalComplex II (succinate dehydrogenase/co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex II catalyzes the oxidation of succinate to fumarate, and in the process reduces ubiquinone (Q) to ubiquinol (QH2). Ultimately,…