Buffers

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  • SDS Protein Gel Loading Solution, 2X is formulated at the traditional 2X stocks, for standard volume of sample to be loaded per well. Simply load the gel sample wells and visualize progress of the electrophoresis run by monitoring the location of the blue dye front. Lane Marker Sample Buffers…

  • 10X PBS, pH 7.4

    Quality Biological

    PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …

  • 1X PBS, pH 7.2

    Quality Biological

    PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …

  • TNE Buffer

    Quality Biological

    TNE Buffer is often used as a base material to generate lysis buffers. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications More specifically, TNE Buffer is useful for: isolation of proteins from yeast cells as a lysis buffer, resuspension of pelleted virus…

  • 10X Protein Running Buffer

    Quality Biological

    Protein Running Buffer, 10X is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE), and is the running buffer for TRIS-glycine gel electrophoresis. Contains 1% SDS Filtered through a 0.22 µm…

  • 10X RNA Gel Loading Solution

    Quality Biological

    RNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989). The purpose of this reagent is to insure that the RNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. Contains two dyes, bromphenol blue…

  • 10X TAE Buffer

    Quality Biological

    Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications …

  • 1M TRIS-HCl, pH 8.0

    Quality Biological

    A hybridization buffer specifically formulated for use with nylon membranes. It does not contain Denhardt’s Reagent, which may interfere with the hybridization reaction of DNA immobilized on nylon. Formulated for Nylon membrane Filtered through a 0.22 µm membrane DNase, RNase, and Protease…

  • 1X TE, pH 8.0

    Quality Biological

    TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. Filtered through a 0.22 µm…

  • DPBS without Ca & Mg, 1X

    Quality Biological

    DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Applications DPBS can be used to provide a buffer system to…

  • 10X TBS, pH 7.4

    Quality Biological

    TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. DNase, RNase and Protease tested. Applications TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can…

  • 10X TBS HCl, pH 7.2

    Quality Biological

    Tris HCl, 10X pH 7.2 is a Buffered Saline Solution used in procedures for the isolation of DNA. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications Tris buffered saline is useful for the following: as a biological diluent buffer, as a buffer for antibodies,…

  • DNA Denaturing Solution

    Quality Biological

    DNA Denaturing Solution is useful for denaturing fractionated DNA prior to the transfer from an electrophoresis gel to nitrocellulose or nylon membrane, as well as Northern and Southern blotting. Contains: 0.5 M NaOH and 1.5 M NaCl Filtered through a 0.22 µm membrane Dnase tested …

  • Enz-Free®

    Quality Biological

    L-glutamine is a cell culture supplement, and an essential amnio acid. L-glutamine is required in cell culture for cellular growth. 100X concentration Applications L-glutamine is used in cell culture as an energy source in addition to carbohydrates (i.e. glucose, sucrose, maltose, etc.).…

  • Kanamycin, 50mg/mL

    Quality Biological

    Kanamycin sulfate is a water soluble broad spectrum antibiotic obtained from soil bacterium Streptomyces kanamyceticus. It is an aminoglycoside antibiotic effective against Gram-negative and Gram-positive bacteria and mycoplasma. It binds to the 30S ribosomal subunit, inhibits translocation, and…

  • 10X PBS, pH 7.2

    Quality Biological

    PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture Applications , such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Applications PBS is also useful in…

  • DPBS with Ca & Mg, 1X

    Quality Biological

    DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Without Phenol red Applications DPBS can be used to provide a…

  • Low-EDTA 1X TE, pH 8.0

    Quality Biological

    Low-EDTA 1X, pH 8.0 is molecular biology grade TE buffer used to store DNA and RNA. EDTA chelates Mg2 and other divalent metals ions – (inhibits DNAse and RNAse to suppress DNA and RNA degradation). Tris is a buffering agent to keep the solution at a defined pH – The ‘Cl’ or ‘HCl’ indicates the…

  • DNA Neutralizing Solution

    Quality Biological

    DNA Neutralizing Solution is used for the isolation of plasmid DNA by alkaline lysis method. Addition of the neutralization solution to lysed bacterial cells adjusts the pH, resulting in precipitation of protein and genomic DNA. Contains 1.5 M NaCl and 1.0 M Tris Filtered through a 0.22 µm…

  • TRIS Glycine Transfer Buffer, 10X (Transblot Buffer, 10X). Applications Tris Glycine Transfer Buffer, at a 1X concentration with the addition of 20% methanol, is used for electrophoretic transfer of proteins from denaturing polyacrylamide gel to a solid membrane – i.e. Western blotting or…

  • 1M Hepes Buffer, pH 7.3

    Quality Biological

    HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent at most biological pH’s and is categorized as an all- purpose buffer available for biological research. pH 7.2 – 7.6 Applications HEPES 1M is used as a buffer alternative from…

  • 2M TRIS-HCl, pH 8.8

    Quality Biological

    Tris HCl 2 M pH 6.8 is useful with SDS-PAGE and Electrophoresis. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). …

  • 1M TRIS-HCl, pH 7.5

    Quality Biological

    Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids. Applications…

  • Formulated as 5X rather than the traditional 2X stocks, SDS Protein Gel Loading Solution 5X enables a larger volume of sample to be loaded per well. 5X concentrated protein gel loading buffer Applications Simply load the gel sample wells and visualize progress of the electrophoresis run by…

  • 5X DNA Gel Loading Solution

    Quality Biological

    DNA Gel Loading Solution, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. …

  • 10X RNA Gel Buffer (10X MOPS)

    Quality Biological

    MOPS Buffer is used at 1X for the electrophoresis of RNA as the running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels. MOPS buffer is also used for Northern Blots -the hybridization of total RNA or mRNA to a membrane. Liquid, 10X concentration Filtered…

  • SSC, 20X

    Quality Biological

    SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization. The concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5X. …

  • This is a special formulation of loading solution for protein gel electrophoresis. Special formulation Applications SDS-PAGE Western Blotting Analysis of DNA samples with high amounts of DNA binding proteins Kinetic experiments DNA agarose gel analysis after DNA restriction digestions,…

  • 10X TBE

    Quality Biological

    TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. Applications EDTA is a chelator of divalent cations,…

  • Sodium Bicarbonate 7.5%

    Quality Biological

    Sodium Bicarbonate (NaHCO3) is typically used in cell culture media as a buffer. Endotoxin and Mycoplasma Tested Applications Although NaHCO3 does not have the buffering capacity of HEPES, it is occasionally preferred over HEPES because of its lower toxicity and nutritional value to cells…

  • Sodium Chloride 0.85%

    Quality Biological

    Sodium Chloride, 0.85% is a saline solution at 0.85% prepared with water used for cell culture. Made with cell culture grade water The sodium chloride is a source of chlorine and sodium necessary to the production of components like sodium carbonate, sodium hydroxide or chlorate ions. …

  • 1X TE, pH 7.5

    Quality Biological

    Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Filtered through a 0.22 µm membrane DNase,…

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