Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run.
Filtered through a 0.22 µm membrane
DNase, RNase, and Protease tested
Applications
TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits. TAE is often prepared in concentrated stock solutions of 10X or 50X in the laboratory. A 1X working solution is prepared prior to electrophoresis.
TAE buffer has a relatively low buffering capacity. Therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended.
Storage Conditions: 15°C to 30°C
Shipping Condition: ambient temperature
Sterile Filtered
DNase RNase and Protease Tested
Concentration: 2 M Tris base and 0.05 M disodium EDTA dihydrate
Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health
Harmonization Code: 3822.00.5090
ECCN: EAR99
Max Shelf Life: 36 months