PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …

Sodium pyruvate is commonly utilized as an additive in culture media and broth as a carbon source, similar to glucose, as pyruvate is the end product of the glycolysis pathway. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We offer a variety of DMEM modifications…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate Without HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…

QBSF-60 is a proprietary serum-free medium that supports the expansion of human hematopoietic stem/progenitor cells. QBSF-60 supports CD34+ cells derived from normal bone marrow, cord blood, peripheral blood stem cells mobilized with cytokines for ex-vivo expansion and differentiation to…

Alpha MEM is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Recommendation This product is made with Earle’s salts. MEM a contains no proteins, lipids, or…

MEM NEAA contains the same non-essential amino acids found in the standard MEM, at a strength of 100. MEM NEAA 100X can be used to enhance cell growth and prolong the viability of cells in culture. 100X of Non-essential amino acids MEM NEAA 100X is commonly used as a supplement to minimal…

Minimize contamination with Endotoxin-Free Water. Eliminate the risk of toxic activity produced by bacteria that can interfere with cell structure and function. Mycoplasma tested DNase, RNase and protease tested Endotoxin < 0.005 EU/mL Applications Ideal for preparing solutions…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…

Terrific Broth is an enriched medium, which helps the recombinant strains of E. coli maintain an extended growth phase. The large amount of tryptone and yeast extract in the medium allows the bacteria to produce a higher plasmid yield. Autoclave sterilized Ready to use Applications …

Sodium Acetate, 3M pH 5.2 is a common molecular biology reagent. All production lots are tested to ensure that they are free of Dnase, Rnase & Proteases. It is often used in buffer systems to maintain the pH of a given solution. Filtered through a 0.22 µm membrane Dnase, Rnase, and Protease…

McCoy’s 5A Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. With L-glutamine and Phenol Red McCoy’s 5A Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a…

QBI's Serum Free Cryo Medium is a sterile, 2X concentrated freezing medium containing DMSO  developed for the cryopreservation of cells grown under serum free conditions. Serum Free Cryo Medium is an animal-free, protein-free, chemically defined medium containing DMSO and other…

YPD/YPED Plates are used for the growth of Saccharomyces cerevisiae (yeast). Applications A solid medium for the propagation of yeast.

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H. Used with SOC medium Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure,…

Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications …

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Applications DPBS can be used to provide a buffer system to…

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. Applications Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223 amino acid residue…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With: High Glucose Without: HEPES Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We…

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture Applications , such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Applications PBS is also useful in…

USDA FBS is used as a supplement in classical cell culture media. USDA FBS both nourishes and protects the cells in a way that almost nothing else has been found to do. USDA Approved – Premium Grade Bovine serum stimulates the cells to grow and multiply and helps to keep the cells…

Potassium Chloride 1 M is used to establish a constant ionic strength in a sample solution. KCl 74.56 g/L Suitable for PCR, molecular biology, protein chemistry and biochemical applications Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications Potassium…

SDS Protein Gel Loading Solution, 2X is formulated at the traditional 2X stocks, for standard volume of sample to be loaded per well. Simply load the gel sample wells and visualize progress of the electrophoresis run by monitoring the location of the blue dye front. Lane Marker Sample Buffers…

Sodium Chloride, 0.85% is a saline solution at 0.85% prepared with water used for cell culture. Made with cell culture grade water The sodium chloride is a source of chlorine and sodium necessary to the production of components like sodium carbonate, sodium hydroxide or chlorate ions. …

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…

A hybridization buffer specifically formulated for use with nylon membranes. It does not contain Denhardt’s Reagent, which may interfere with the hybridization reaction of DNA immobilized on nylon. Formulated for Nylon membrane Filtered through a 0.22 µm membrane DNase, RNase, and Protease…

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. Applications ACK Lysing Buffer is used to lyse red blood cells in samples, such as EDTA-treated…

Penicillin-Streptomycin acts in two ways. Penicillin inhibits bacterial cell wall synthesis. While, streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding. 100X concentration of mixture of penicillin…

Compared to other basal media, Ham’s F-12 contains a wider variety of components, and no proteins or growth factors. Without hypoxanthine and L-glutamine Ham’s F-12 uses a sodium bicarbonate buffer system (1.176 g/L); therefore requiring a 5–10% CO2 environment to maintain physiological pH. …

Fischer’s medium supports clonal growth of cells – particularly lymphoblasts – from primary explants or cells already in culture. With L-glutamine and Phenol Red Applications Fischer’s medium also supports mammalian cell culture and murine hematopoietic cells. Recommendation …

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. Ready to use solution Applications Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis…