His Elution Buffer for Immobilized Metal Affinity Chromatography. Suitable for our Immobilized Cobalt , Copper , Nickel , and Zinc chelating resins Features 250mM Imidazole, 300mM NaCl, 50mM sodium phosphate (pH8.0) Immobilized Cobalt , Copper , Nickel , and Zinc chelating resins are also available