SpinOUT™ centrifugal filter devices offer rapid and efficient concentration and purification of biological samples. The vertical design allows for rapid sample processing and high sample recovery rate (typically greater than 90% of dilute starting solution), while maintaining gentle conditions to preserve biomolecule activity and conformation. The vertical design minimizes solute polarization and subsequent fouling of the membrane and a physical deadstop in the filter device prevents spinning to dryness, resulting in sample loss. A wide selection of ultrafiltration molecular weight cut-off (MWCO) devices incorporating a PES membrane provide minimal protein and nucleic acid binding.
FEATURES
• High sample recovery
• Fast sample flow
• Low bio-molecule binding rate, high chemical compatibility
• Deadstop design prevents damage to samples caused by over-centrifuging
• Wide variety of Molecular Weight Cut Off membranes
ULTRA-FILTRATION CENTRIFUGAL FILTER (5 – 100 kDa MWCO)
• Concentrate and desalt proteins, nucleic acids
• Buffer exchange or desalting of chromatographic components
• Harvest bio-molecules from culture media
• Virus concentration or isolation
• Coarse separation of bio-molecule mixtures
MICRO-FILTRATION CENTRIFUGAL FILTER (0.22 uM- 1.0 uM pore size)
• Isolate DNA from agarose gel
• Separation of proteins, oligonucleotides and RNA from polyacrylamide gels
• Sample filtration before HPLC analysis
• Media removal of cells
• Filter biological samples
• Collect and clean processed particles or beads