Percoll® is a medium for density gradient centrifugation of cells, viruses and subcellular particles. Percoll® is composed of colloidal silica coated with polyvinylpyrrolidone (PVP), which renders the material completely non-toxic and ideal for use with biological materials. Centrifugation…

CELLect Gold is collected from a U.S.D.A. regulated and monitored abattoir by technicians who work "on-site" where the fetal blood is aseptically collected via cardiac puncture. This raw material is transported to the production facility where it undergoes sterilization via filtration…

Glycerol is a simple polyol compound. Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid…

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. This solution contains 17,000 - 21,000 units/mL of penicillin and 18.0 - 23.0 mg/mL of streptomycin. …

RPMI 1640 was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also…

Tween 80 is a polyethylene sorbitol ester, non ionic surfactant and an emulsifier derived from polyethoxylated sorbitan and oleic acid. Tween 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture, growing of tubercule…

Hydrochloric acid is used as a laboratory reagent, for pickling of steel, in production of organic and inorganic compounds, for pH control and neutralization, used in the regeneration of ion exchange resins, in the production of food, food ingredients, and food additives. Chlorine source for safer,…

Isopropyl alcohol is widely used in chemistry, biochemistry and molecular biology. Industrial applications include its use in antifreezes, quick-drying oils, and quick-drying inks, and as a solvent for gums, shellac, and essential oils. Isopropyl alcohol has been utilized as a solvent in the…

DNA-Erase™ is a non-alkaline, non-corrosive and non-carcinogenic cleansing solution that is highly active against DNA contamination and is intended for use at PCR workstations for eliminating PCR artifacts from all laboratory surfaces, including benchtops, apparatus and pipettors. Presence of…

Preparation Method: Add 5% less distilled water than the desired total volume to a mixing container just large enough to hold the final media volume. Add powdered medium to room temperature (20 -30°C) water with gentle stirring. Do not heat water. Rinse inside of pouch to remove all traces of…

FastGro allows culturing cells in vitro without the use of serum and without any animal or human derived compound. FastGro is a fully defined additive, avoiding the need for serum, and used for a wide range of cell culture practices. Since FastGro is chemically defined, it avoids lot-to-lot…

Donor Horse Serum is suitable for use in diagnostic assays, as a supplement in mycoplasma growth and assay media and for culturing hematopoietic stem cells and neuronal cells. This serum is processed from whole blood collected from a controlled herd of young horses. Each animal is routinely…

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein…

Polyethyleneimine is a branched chain polymer having a ratio of 1:2:1 of primary:secondary:tertiary amines with a branching site every 3 to 3.5 nitrogen atoms and a base structure of (CH₂CH₂NH)x. The nitrogen atoms must be protonated to achieve cationic charge, so the cationic…

Fetal Bovine Serum (FBS) is the growth supplement of choice for cell culture due to its high level of nutrients. Most cell types and cell culture systems will benefit from the inclusion of FBS in growth media. It is produced to the highest standards to ensure product performance and safety with…

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals. Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to…

Benzyl alcohol (BnOH) is a monoaromatic primary alcohol widely used as a solvent and an intermediate in cosmetic formulations, pharmaceutical and flavor/fragrance industries. It shows bacteriostatic property. Commercial method for its synthesis is by the alkaline hydrolysis of benzyl chloride.…

Polysorbate 20 is typically used as an emulsifying agent for the preparation of stable oil-in-water emulsions, particularly in pharmaceutical applications. It is also used in pre-extraction of membranes to remove peripheral proteins & as a blocking agent on nitrocellulose at a typical…

Acetic anhydride is a reagent that is used in the manufacturing of acetyl compounds and cellulose acetates. It is also utilized as a solvent in the examination of wool fat, glycerol, fatty and volatile oils, and resins. In the synthesis of organic compounds, acetic anhydride is used in various…

Triton® X-100 is a alkylaryl polyether alcohol. It is a nonionic surfactant prepared by the reaction of octylphenol with ethylene oxide. The number ("-100") relates only indirectly to the number of ethylene oxide units in the structure. The principal uses of Triton® X-100 is…

Calcium and magnesium support cell adhesion. Hanks' Balanced Salts (Modified), without Magnesium and Calcium, is used to minimize cell attachment or aggregation.

Hydrogen peroxide is used as a reagent, disinfectant, antiseptic, and propellant. In synthetic chemistry, H2O2 is used for epoxidation and hydroxylation transformations, and is used as the raw oxidant source for generating more complicated and specific organic/inorganic peroxides.

2-Merceptoethanol is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. 2-Merceptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis. 2-mercaptoethanol is readily oxidized in air to a disulfide,…

Eosin Y, a red xanthene dye, has been noted to reduce the affinity of Mg2+- ATPase for ATP and increase the enzyme affinity for Mg2+ at concentrations of 10-50 μM. At higher concentrations near 100 μM the affinity for Mg2+- ATPase for the ion-activator has been shown to be reduced. This…

Water is the most fundamental and universal solvent known in nature. Both the polar character and the hydrogen bonding capability of water contribute to its unique solvent properties. Polar materials dissolve in water readily. In the case of salts, water can readily overcome the electrostatic…

Liqui-Gel is a 40% (w/v) solution of Ultra Pure Acrylamide (38.96%) and bis-Acrylamide (1.04%) in specially-prepared deionized water. Final ratio is 37.5:1. Acrylamide decomposes in the presence of acids, bases, oxidizing agents, iron, and iron salts. It decomposes non-thermally to form…

Dimethyl sulfoxide is a dipolar, aprotic solvent. Has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination. Dimethyl sulfoxide is used to enhance dermal absorption of many chemicals. A solvent for many…

Goat serum is a suitable alternative to fetal bovine serum in a wide range of cell culture applications such as the isolation and identification of viruses produced in a variety of primary cell lines. This serum is produced from whole blood collected from healthy adult animals.

2-8 CELLsium is a cytoprotective, protein free, ready-to-use bio-solution without Dimethyl Sulfoxide (DMSO) for the short-term storage of cells or tissue. For short-term storage, add cold (2-8 °C) 2-8 CELLsium at a recommended cell concentration of 1 x 10 6 mL to 8 x 10 6 mL. The 2-8 CELLsium…

Formaldehyde is used as bactericidal agent. Formaldehyde is used as an embalming agent due to its ability to crosslink primary amino groups in proteins through a methylene linkage; however, due to its extremely toxic nature it tends to be avoided. Formaldehyde is also used in numerous industrial…

Protexidase buffer is 500 mL of PBS, pH 7.2, containing 0.5% BSA, antimicrobial preservatives and proprietary stabilizing compounds. This product stabilizes high dilutions of HRP-antibody conjugates, up to 1:16,000 dilution and inhibits microbial growth in dilute conjugates. This protective…

BCIP/NBT Liquid Substrate Plus is a stabilized chromogen solution. It has a greater sensitivity and produces little or no background staining compared with other alternatives. It is a ready to use solution. The BCIP/NBT Liquid Substrate System offers a convenient, ready-to-use substrate solution…