BCIP/NBT Liquid Substrate Plus is a stabilized chromogen solution. It has a greater sensitivity and produces little or no background staining compared with other alternatives. It is a ready to use solution.
The BCIP/NBT Liquid Substrate System offers a convenient, ready-to-use substrate solution for visualizing alkaline phosphatase activity in immunoblotting and immunohistology. Immunohistochemical staining with BCIP/NBT requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green.
BCIP/NBT [5-bromo-4-chloro-3-indolyl-phosphate p-nitro blue tetrazolium chloride] is in a proprietary buffer and enhancer.
BCIP/NBT Liquid Substrate Plus apply to the membrane and allow the color to develop. Pour off and rinse with distilled water to stop the reaction.
Concentration: BCIP: 0.692 mmol/liter; NBT: 0.734 mmol/liter
Key Applications: Immunohistology, Immunoblotting
Application Areas: Immunoassays
Product Type: Reagents
Format: Liquid
pH: 9.8 [+/- 0.1]
Storage & Handling: Store product at +2-8°C