Mitomycin C is the most widely used of the Mitomycin antibiotics because it is the least toxic and with the most potent antitumor activity. Inhibitor of DNA synthesis, nuclear division, and cancer cells. Antibacterial to gram positive, gram negative, acid-fast bacilli. Mitomycin C…

Suitable for cell adhesion in tissue culture, as well as gel formation, platelet aggregation, and as a substrate for collagenase. Collagen is a group of naturally occurring proteins found in animals, especially in the flesh and connective tissues of vertebrates. It is the main component of…

Bleomycin Sulfate is an atineoplastic antibiotic. A mixture of cytotonic glycopeptide antibiotics which reacts with DNA and causes strand scission; they have also been shown to have a type of oxygen transferase activity. Bleomycin A2 is the main component of the bleomycin employed clinically.…

Tunicamycin is a mixture of Tunicamycins A, B, C and D. It is a nucleoside antibiotic active in vitro against Gram-positive bacteria, yeasts, fungi, and viruses. Tunicamycin has been used to study the effect of N-linked glycosylation of human proton-coupled folate transporter (HsPCFT) in HeLa…

Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolized in the tricarboxylic acid cycle to produce…

Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When…

Guanosine-5'-triphosphate, a purine nucleotide, is required as a coenzyme for protein biosynthesis in a cell-free system. Guanosine 5′-triphosphate (GTP), a purine trinucleotide, is a substrate for RNA polymerases and a wide range of GTPase including G-protein coupled receptor (GPCR)…

Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding. A compromise between the easier to use lower molecular weight products…

Heparin is a polymer classified as a mucopolysaccharide or a glycosoaminoglycan. It is biosynthesized and stored in mast cells of various mammalian tissues, particularly liver, lung and mucosa. It is made up of repeated disaccharide units consisting of 1,4-linked L-iduronic acid and D-glucosamine.…

Poly-L-lysine is a positively charged amino acid polymer. Poly-lysine binds to DNA, red cell membrane and any negatively charged protein. It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell…

Citrinin is a fungal metabolite found in moldy rice. Citrinin was used to evaluate toxicity of the mycotoxin citrinin using yeast ORF DNA microarray and Oligo DNA microarray.

Propidium iodide reagent is used for the fluorescent staining of nucleic acids. Propidium iodide (PI) binds to DNA by intercalating between the bases with little or no sequence preference and with a stoichiometry of one dye per 4-5 base pairs of DNA. PI also binds to RNA, necessitating treatment…

Human chorionic gonadotropin (HCG), a polypeptide hormone produced by the human placenta, is composed of an alpha and a beta sub-unit. The alpha sub-unit is essentially identical to the alpha sub-units of the human pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone…

Puromycin is an antibiotic of unique structure and biological activity. It is produced by fermentation using Streptomyces albo-niger. There are four aspects of puromycin's biological activity that are 1) antitumor effect; 2) nephrotoxic action; 3) inhibition of purine and/or protein synthesis…

Elastase is an enzyme from the class of proteases (peptidases) that break down proteins. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids. Hydrolyses elastin Digests hemoglobin, casein, and fibrin One unit will solublize 1 mg of elastin in 20 minutes at…

Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. Ribonuclease A is used to remove RNA from DNA plasmid preparations…

Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The…

Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. Molecular weight has been determined by Viscosity as well as by HPLC. It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction…

Phosphatidylserine is a phospholipid component, usually present on the cytosolic side of cell membranes by an enzyme called flippase. When a cell undergoes apoptosis, phosphatidylserine is no longer restricted to the cytosolic part of the membrane, but becomes exposed on the surface of the cell. …

Aprotinin is a single chain polypeptide (58 amino-acids) crosslinked by three disulfide bridges, showing competitive and reversible inhibiton of proteolytic and esterolytic activity. Aprotinin forms stable complexes with, and blocks the active sites of serine protease enzymes. It is found in bovine…

A metabolite with a broad antibiotic spectrum. Inhibitor of mitochonclrial action. K+-selective ionophoric cyclodepsipeptide; potassium ionophore which uncouples oxidative phosphorylation, induces apoptosis in murine thymocytes, inhibits NGF-induced neuronal differentiation and antagonizes…

Uridine-5'-diphosphogalactose Disodium Salt is a donor substrate for galactosyltransferases involved in the addition of galatose (galactosylation) molecules to N-linked and O-linked oligosaccharides and glycerolipids(biosynthesis of galactose-containing oligosaccharides). UDP-Gal and its…

Angiotensin II is a peptide hormone which plays an important role in the regulation of blood pressure. It stimulates the release of aldosterone from the adrenal gland. It has strong vasoconstrictive effects. Angiotensin II plays an important role in the regulation of blood pressure. It…

An antineoplastic of Rhodomycin group; Antibiotic; Inhibitor of enzymes and metabolic pathways. A potent anticancer agent that has been shown to be a strong inhibitor of DNA and RNA synthesis. Daunorubicin hydrochloride is used in photostability , antileukemic, and drug metabolism studies. …

Coenzyme A is an essential cofactor in enzymatic acetyl transfer reactions. Facilitates removal of lipid peroxides by increasing mobilization of fatty acids Promote repair of plasma membranes by activating phospholipid synthesis The principal biologically active forms of pantothenic…

Actinomycin D is a polypeptide antibiotic first described by Waksman. It is bacteriostatic in nature, especially towards gram negative bacteria, and also has anti-tumor activity. Actinomycin D has a wide-ranging applications ranging from a selection agent in cell culture, use in studies of…

Avidin is a basic glycoprotein consisting of four essentially identical subunits. The combined molecular weight of the subunits is about 66,000. Each subunit is a single polypeptide chain containing 128 amino acid residues with alanine at the N-terminal, glutamic acid at the C-terminal, and a…

Lucifer Yellow CH is the hydrazine (-NH-CO-NH-NH2) form of the dye compared to Lucifer Yellow VS, which is the vinyl sulfone (-Ph-SO2-CH=CH2) form. It is a highly fluorescent dye useful in marking nerve cells. Lucifer yellow CH has been used to demonstrate electrical coupling between hamster…

Arginine-rich subgroup. Commonly used for chromosome-reconstitution studies. Suitable substrate for protein kinase C.

Muscimol is a structural analog of γ-amino butyric acid. It functions as a GABA-agonist: a positive analog of the natural transmitter, which can activate GABA receptors in the brain. Muscimol is an isoxazole derivative occurring naturally in the mushroom Amanita muscaria. Muscimol has been…

Limonin is a natural compound found in grapefruit and other citrus seeds. Limonin is a triterpenoid aglycone that is a bitter principle of citrus fruits. It has been shown to inhibit chemically induced carcinogenesis and demonstrates anti feedant properties. Limonin may be used in assays to…

Acetoacetyl coenzyme A (Acetoacetyl-CoA) may be used as a substrate for a number of enzymes including; acetoacetyl-CoA thiolase (EC 2.3.1) which produces acetyl-CoA in reverse condensation reaction and (3-Hydroxy-3-methylglutaryl coenzyme A (CoA) synthase (HMGCS)) which produces…