Fungus-Derived Proteinase K Purified Recombinant is prepared at 20 mg/mL in 10 mM Tris-HCl, 1 mM calcium acetate, pH 7.5 containing 50% glycerol. This Molecular Biology Grade Proteinase K solution is extremely effective on native proteins and can be used to rapidly inactivate endogenous nucleases…

Goat serum is a suitable alternative to fetal bovine serum in a wide range of cell culture applications such as the isolation and identification of viruses produced in a variety of primary cell lines. This serum is produced from whole blood collected from healthy adult animals.

Hydrogen peroxide is used as a reagent, disinfectant, antiseptic, and propellant. In synthetic chemistry, H2O2 is used for epoxidation and hydroxylation transformations, and is used as the raw oxidant source for generating more complicated and specific organic/inorganic peroxides.

Isoamy alcohol is routinely used in molecular biology, especially in the purification of DNA. It is widely used in conjunction with phenol and chloroform (25:24:1 phenol : chloroform : isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction. Isoamyl alcohol is…

Hexadecane (n-Hexadecane) is an aliphatic long-chain hydrocarbon. It is a reference fuel with the cetane number 100 and is employed in determining the cetane number of diesel fuels. The mechanism of the oxidation of hexadecane in the gas-phase has been described. The density, speed of sound,…

Polyethyleneimine is a branched chain polymer having a ratio of 1:2:1 of primary:secondary:tertiary amines with a branching site every 3 to 3.5 nitrogen atoms and a base structure of (CH₂CH₂NH)x. The nitrogen atoms must be protonated to achieve cationic charge, so the cationic…

Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc. Tween® 80 has been widely used…

2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its…

Rabbit serum is a component of blood obtained from a rabbit that contains blood plasma without fibrinogen or other clotting factors. Rabbit Serum Combine with BSA to use as a blocking serum in immunocytochemistry and immunohistochemistry. In immunohistochemistry, antibodies are used to detect…

Stabilized Chromogen Solution - This alkaline phosphatase chromogen solution contains the two components in a stabilized solution which may be used as the final step in color development. The resulting product is a permanent, deep-purple stain. It enhances results in both ELISA and immunohistology…

Catalase, Fungal suspension from from Aspergillus niger is long-acting, extremely stable form of catalase that is active over a wide pH range. Composed of four protein subunits Each subunit contains a heme group bound to its active site Catalase is used for the removal of peroxides,…

Trypsin-EDTA solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation due to its digestive strength.

Triton® X-100 is a alkylaryl polyether alcohol. It is a nonionic surfactant prepared by the reaction of octylphenol with ethylene oxide. The number ("-100") relates only indirectly to the number of ethylene oxide units in the structure. Triton X-100 is a non-ionic surfactant and…

HFBA is a strong acid and an ion-pairing agent. The strong acidity of HFBA ensures that other acidic groups such as carboxylic acid moieties on biomolecules remain protonated, and thus the samples are able to interact with organic solvents in such processes as reverse phase chromatography. The…

A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with approximately one-half mole equivalent of sodium hydroxide or potassium hydroxide to the desired pH. A simple mixing table for preparing 0.05 M HEPES/NaOH has been…

Tween 80 is a polyethylene sorbitol ester, non ionic surfactant and an emulsifier derived from polyethoxylated sorbitan and oleic acid. Tween 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture, growing of tubercule…

Donor Horse Serum is suitable for use in diagnostic assays, as a supplement in mycoplasma growth and assay media and for culturing hematopoietic stem cells and neuronal cells. This serum is processed from whole blood collected from a controlled herd of young horses. Each animal is routinely…

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein…

Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.

Isopropyl alcohol is widely used in chemistry, biochemistry and molecular biology. Industrial applications include its use in antifreezes, quick-drying oils, and quick-drying inks, and as a solvent for gums, shellac, and essential oils. Isopropyl alcohol has been utilized as a solvent in the…

Ammonium acetate is a chemical compound which is derived from the reaction of ammonia and acetic acid. Ammonium acetate is volatile at low pressures. Because of which it has been used to replace cell buffers with non-volatile salts, in preparing samples for mass spectrometry. It is used as a buffer…

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium…

Trichloroacetic acid is an acetic acid analogue commonly used to precipitate proteins, DNA and RNA. In the presence of SDS Trichloroacetic acid will precipitate proteins which can then be quantified by the Lowry method. The compound has also found uses as a decalcifier and fixative in microscopy,…

L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly.…

Suitability: Suitable for cell culture Key Applications: Cell culture media supplement Product Type: Biochemicals Chemical Class: Antibiotics

Dimethyl Sulfoxide is a dipolar, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination. Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for…

IGEPAL™ CA-630 is a homologous series of octylphenoxypoly(ethyleneoxy)ethanols used for the solubilizing and isolation of membrane proteins. The Igepal CA surfactants are all derived from the same hydrophobic starting material, octylphenol. By increasing the amount of hydrophilic…

Hexane is used in laboratory to extract oil and grease contaminants from water and soil for analysis.

Mineral oil is used as an overlay covering microdrops of culture medium in petri dishes, during the culture of oocytes and embryos in IVF and related procedures. It also has its applications in food processing, cosmetics and pharmaceuticals. A mineral oil is any of various colorless, odorless,…

Phenol red is an acid-base indicator. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Phenol Red is used as a pH indicator in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a…

TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C). At temperatures above the cloud point, detergent solutions separate into aqueous and detergent-enriched phases. This makes Triton X-114 particularly useful in…

Glycerol is a simple polyol compound which is a viscous liquid that is widely used in pharmaceutical formulations. It has three hydroxyl groups that are responsible for its solubility in water and its hygroscopic nature. It is a trihydroxy sugar alcohol that is an intermediate in carbohydrate and…