Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.
Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:
- Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance
- Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
- Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
- Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)
Suitability: Suitable for cell culture
Key Applications: Used for the handling and culturing of mammalian cells
Application Areas: Handling and culturing of mammalian cells.
Product Type: Reagents
Presentation: Clear, Colorless Solution
Format: Solution
Formulation: Inorganic Salts: Potassium Chloride [KCl]: 200.00 mg/L; Potassium Phosphate Monobasic [KH2PO4]: 200.00 mg/L; Sodium Chloride [NaCl]: 8000.00 mg/L; Sodium Phosphate Dibasic [Na2HPO4]: 1150.00 mg/L
pH: 7.0 - 7.6
NOTES: Osmolality: 270 - 305 mM/kg
Sterility: Sterile
Storage & Handling: Store at Room Temperature (15-30°C).