Corning Tissue Culture Media
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RPMI 1640
CorningNamed for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse…
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Leibovitz's L-15
CorningLeibovitz's L15 medium was originally developed without a sodium bicaronate buffer system for use in non-CO2 equilibrated environments. It is buffered by phosphates and the amino acid L-arginine, along with L-histidine and L-cysteine. The formation of acid metabolite byproducts is prevented by…
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Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. in vitro use of collagen can exert effects on the…
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Lymphocyte Serum-Free Medium
CorningCorning's line of specialty media is optimized for those who require media for a wide range of specialized applications and cell lines. The use of T-lymphocytes in adaptive immunotherapy is emerging as an important tool in the treatment of cancer and other clinical applications.…
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Yeast Extract
CorningYeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates obtained from autolyzed yeast. This is the water soluble portion with intact B-complex vitamins and can be used as an additive for culture media. 10,000 MW cut-off
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Corning BioCoat cell culture inserts are pre-coated with extracellular matrix proteins for applications requiring a protein-coated cell surface, such as cell adhesion, growth, invasion, migration and/or differentiation. Coatings include Corning Matrigel® matrix, Fibronectin, Collagen, and Laminin.
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Corning® Cell Recovery Solution
CorningAllows for recovery of cells cultured on Corning Matrigel Matrix. It depolymerizes Matrigel without enzymatic digestion and lengthy incubation periods at high temperatures for subsequent culturing and/or biochemical analysis of cells. Used to depolymerize Matrigel matrix in 2D or 3D cultures,…
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A 96-well insert system with a filter plate pre-coated with structured layers of phospholipids. Design and Materials Microplate type: 96-well microplate configuration complies with ANSI standards Filter plate: Polystyrene with 0.4 µm Polyvinylidene fluoride (PVDF) membrane …
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Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN…
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Type IV Collagen is a ubiquitous component in basement membranes and provides the major structural support for this matrix. When the Collagen IV meshwork is assembled, it provides a scaffold for the assembly of other basement membrane components through interactions with laminin, entactin/nidogen,…
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Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6. It can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of…
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Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
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Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN…
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Thomas No. B003K43 A modified forumulation of Minimal Essential Medium, Improved Minimum Essential Medium (IMEM) contains a higher concentration of many components used in MEM along with additional amino acids, vitamins, and metabolites. Complete Certificate of Analysis available…
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Optimized for the cultivation of Chinese Hamster Ovary (CHO) cells, along with many derivatives of this parent line, Corning FREE™ is a proprietary serum-free and protein-free growth medium that does not contain any hormones or growth factors. This medium supports superior growth and…
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Interleukin-2 (IL-2), mouse recombinant, plays a central role in cell-mediated immune response. Was first identified by its ability to promote the long-term proliferation of activated T-cells. (25,000 BRMP units) References: 1. Hatakeyama, M. and Taniguchi, T., "Interleukin-2" in Peptide Growth…
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Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
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Amino Acids & Vitamins
CorningL-glutamin L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds.…
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Corning® 500 mL RPMI 1640, 1X
CorningCorning glutagro™, phenol red Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy’s 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and…
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HTS insert plates are arrays of individual cell culture inserts connected by a rigid, robotics-friendly holder. This single-unit design makes insert plates ideal for running automated, high throughput drug transport (Caco-2 cells) cell toxicity studies or cell migration and invasion…
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Poly-D-Lysine, 20 mg
CorningPoly-D-Lysine, used to culture a wide variety of cell types, particularly neurons, glial cells, and transfected cells. (20 mg) Source: Synthetic Quality: Shown to promote cell attachment and spreading of rat cerebellar granule cells Filtered (0.2 µm membrane) and tested and found negative…
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Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
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Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN…
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Cell Dissociation
CorningTrypsin is used to enzymatically release adherent cells from tissue culture plates for passaging. Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action. EDTA sequesters these ions and thus enhances the efficacy of…
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Corning extracellular matrices (ECMs) enable researchers to mimic in vivo environments for 2D and 3D cell culture applications.
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This Tryptic Soy Broth (TSB) product is offered for use in sterility testing and validation in the manufacture of a liquid product. The use of this medium in place of the product intended for production allows an accurate assessment of sterility throughout the manufacture process. It is a sterile,…
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For some applications, the use of a combination of ECM proteins, such as Laminin (LM) and Fibronectin (HFN) or LM and attachment factors such as Poly-D-Lysine (PDL) or Poly-L-Orinthine (PLO) has been shown superior to the use of either alone. Corning BioCoat PDL/LM and PLO/LM Cultureware is…
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Laminin (LM), a major component of basement membranes, is a multifunctional glycoprotein that is used as a substrate to culture and maintain differentiated function of a wide variety of cells. Laminin has been shown in culture to stimulate neurite outgrowth, promote cell attachment, chemotaxis,…
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Corning® Ham's F-12 Medium
CorningHam's F-12 media was originally formulated for the serum-free culture of CHO cells, and is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids. This medium is also suitable for carcinoma cells, rat skeletal myoblasts,…
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Corning insectagro Sf9 is formulated to support the propagation of Sf9 insect cells in culture, and can also be used for SF21 cells. The Sf9 cell line originated at the USDA Insect Pathology Laboratory from IPLBSF-21 cell line, derived from the pupal ovarian tissue of the fall army worm. Sf9 cells…
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Trace Elements A, B, and C
CorningTrace Elements A provides copper, zinc, iron, and selenium. Selenium aids in the detoxification of free radicals as a cofactor for GSH synthetase, while iron, copper, and zinc may be bound by serum protein. The combination of Trace Elements A contains the trace elements of MCDB 104 and of MCDB…