Interleukin-2 (IL-2), mouse recombinant, plays a central role in cell-mediated immune response. Was first identified by its ability to promote the long-term proliferation of activated T-cells. (25,000 BRMP units)
References:
1. Hatakeyama, M. and Taniguchi, T., "Interleukin-2" in Peptide Growth Factors and Their Receptors I, Springer-Verlag, NY (1990).
2. Kashima, N., et al., Nature 313:402 (1985).
3. Leonard, W.J., et al., Proc. Natl. Acad. Sci. USA 80:6957 (1983).
4. Mori, H., et al., Int. Immunol. 3:149 (1991).
5. Morgan, D.A., et al., Science 193:1007 (1976).
6. Smith, K.A., Science 240:1169 (1988).
7. Meidema, F. and Melief, J.M., Immunol. Today 6:258 (1985).
8. Triachiesi, T., et al., J. Exp. Med. 16:1147 (1984).
9. Grimm, E.A., et al., J. Exp. Med. 155:1823 (1982).
Formulation:
Frozen in Dulbecco?s PBS, containing 0.1% BSA.
Preparation and Stoage:
Stable for = three months at -70°C. Avoid multiple freeze-thaws.
Quality:
- Purity > 95% by SDS-PAGE
- Tested for ability to stimulate proliferation of the mouse T-cell line CTLL
- Filtered (0.2 µm membrane) and tested for bacteria, fungi and mycoplasma
Source:
Recombinant, murine T-cell line
Molecular Weight:
17 kD
Tip:
Recommended concentration - 10 BRMP units/ml of medium depending on cell type.