Tissue Culture Media

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  • RPMI 1640

    Corning cellgro

    Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse…

  • A less complex and extensively modified version of Medium 199, CMRL-1066 was designed initially for use with L-strain cells in unsupplemented culture. It has been used to maintain monolayer growth of permanent cell lines for years without protein supplementation. Although developed for use in…

  • Hanks' Balanced Salt Solution 10X w/ Phenol red w/o Sodium bicarbonate. Used with cells maintained in less CO2 environment or CO2 free environment.

  • Sodium bicarbonate is the most commonly used biological buffer in cell culture media and reagents. It is typically added to cell culture media to maintain the physiological pH of a culture and to avoid undesirable fluctuations of pH due to cell metabolism that may have negative effects on cell…

  • Cefinase™ discs are intended for use in rapid testing of isolated colonies of Neisseria gonorrhoeae, Staphylococcus species, Haemophilus influenzae, enterococci and anaerobic bacteria for the production of β-lactamase.

  • McCoy's 5A medium was developed at Roswell Park Memorial Institute in Buffalo, New York. The first medium was developed in 1955 as the result of studies on the nutritional requirements of the Walker 256 carcinoma. The original formulation was based on the amino acids in concentrations similar to…

  • Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of…

  • Glutamine XL™, 200mM

    Quality Biological

    Applications Standard, unstabilized solutions of L-glutamine spontaneously break down over time to release cytotoxic amines. Glutamine XL is a stabilized solution that prevents degradation and cytotoxicity, with the benefit of a long shelf life. The incorporated L-alanine protects the unstable…

  • Use Cytiva HyClone 100 mM Sodium Pyruvate solution, an easily accessible carbohydrate source that may also have protective effects against hydrogen peroxide.

  • Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. It also plays a key role in mast cell development, gametogenesis, and melanogenesis. Tip: Recommended concentration - 0.2-20…

  • Tumor Necrosis Factor-a (TNF-a) has growth-inhibitory effects on many tumor cells and nontumor cells, e.g., certain hematopoietic cells. It also has differentiation-inducing effects, e.g., on macrophages. Source: Recombinant, E. coli Quality: Purity =97% by SDS-PAGE Tested for ability…

  • Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation. MEM is modified with higher…

  • This PBS formulation is recommended for use in immunohistology protocols. Each package makes 1L of 10 concentrated PBS or 10 liters of 1 x solution. Each 1 x PBS solution contains 137 mM Sodium Chloride, 2.7mM Potassium Chloride and 10mM Phosphate Buffer, pH 7.3-7.5 (1.0%, Water) at 25°C.

  • CDC Anaerobe 5% Sheep Blood Agar is used for the isolation and cultivation of fastidious and slow growing, obligately anaerobic bacteria from a variety of clinical and nonclinical materials. It also supports good growth of most aerobic, facultatively anaerobic and microaerophilic bacteria if…

  • Modified Barth's Solution (MBS) is used forstorage and maintanance of oocytes from Xenopus laevis. Product information autoclaved Use immediately once opened. Store at 4 - 8 °C Ional composition of MBS Solution NaCl = 88 mM KCl = 1 mM CaCl2 = 0,4 mM Ca(NO3)2 = 0,33…

  • Thomas Nos. B003K38 and B003K39 Iscove's Modification of DMEM (IMDM) is highly enriched synthetic medium optimized for rapidly proliferating cultures of high cell density.  A modified form of Dulbecco's Modified Eagle Medium (DMEM), Iscove's contains additional amino acids,…

  • Cell Freezing Medium A

    Quality Biological

    Cell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend…

  • The 30° angled cassette features an extra-large writing surface and two side panels ideal for labeling. The cassette has an integral lid that snaps apart and securely locks into the base of the cassette. The lid can be removed and reattached repeatedly to access the specimen. Note: These cassettes…

  • The 30° angled cassette features an extra-large writing surface and two side panels ideal for labeling. The cassette has an integral lid that snaps apart and securely locks into the base of the cassette. The lid can be removed and reattached repeatedly to access the specimen. Note: These…

  • Corning BioCoat cell culture inserts are pre-coated with extracellular matrix proteins for applications requiring a protein-coated cell surface, such as cell adhesion, growth, invasion, migration and/or differentiation. Coatings include Corning Matrigel® matrix, Fibronectin, Collagen, and Laminin.

  • Detach and disaggregate adherent cells from the surface or substrate to which they are attached with Cytiva HyClone Trypsin Protease. Derived from porcine pancreas Gamma irradiated prior to hydration and filling Formulated without calcium and magnesium

  • Corning BioCoat Gelatin cultureware provides an attachment and growth promoting substrate for the culture of a variety of cell types. Gelatin is commonly used in the culture of vascular endothelial cells, muscle, embryonic stem (ES) cells, and F9 teratocarcinoma cells. It is also suitable for…

  • Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN…

  • Accutase® cell detachment solution contains proprietary marine-origin enzymes with proteolytic and collagenolytic activity for the detachment of primary and stem cell lines and tissues. It’s useful for the routine detachment of cells from standard tissue culture plasticware and adhesion…

  • The Corning portfolio of cytokines, growth factors, and media supplementation offers a wide array of proteins that result in proliferation or differentiation of the affected cells. Vialed, highly purified growth factors with high biological activity Broad range of highly purified natural and…

  • Support cell culture growth and biomanufacturing processes with Cytiva HyClone RPMI 1640 media, manufactured using ISO 9001 certified processes.

  • Dulbecco's Phosphate Buffered Saline 1X w/ Calcium and Magnesium w/o Phenol red. Commonly used for tissue disaggregation and monolayer dispersal.

  • Platelet-Derived Growth Factor-BB (PDGF-BB), human recombinant, a dimeric glycoprotein composed of two A and/or B chains, is the principal mitogen inserum for mesenchymal cells. (10 µg). Formulation: Lyophilized from 10 mM acetic acid, carrier-free; reconstitute in 10 mM acetic acid or…

  • DMEM with Glutamine XL

    Quality Biological

    DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With Stabilized L-glutamine Applications DMEM is one of the most widely used modifications of Basal Medium Eagle (BME). DMEM was originally developed as a…

  • Kanamycin is used to select for mutated or transformed cells that are kanamycin resistant. In research labs, there are several commonly used kanamycin resistance genes (e.g., aph, ntpII , and aaC-aphD ). Typically, low-copy-number plasmids require less kanamycin than high-copy-number plasmids. …

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