Poly-D-Lysine, used to culture a wide variety of cell types, particularly neurons, glial cells, and transfected cells. (20 mg)
Shown to promote cell attachment and spreading of rat cerebellar granule cells Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, and mycoplasma
Preparation and Storage:
Stable for three months at 2° to 8°C (lyophilized) or three months at -20°C (solubilized). Avoid multiple freeze-thaws. kD
Recommended concentration - 2.5-5 µg/cm2 of growth surface depending on cell type (guidelines for coating included with product).
Lyophilized from aqueous solution; reconstitute in dH20