Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. in vitro use of collagen can exert effects on the adherence, morphology, growth, migration, and differentiation of a variety of cell types. Applications include:Promotion of cell attachment and spreadingRapid expansion of cell populationsSerum-free or reduced serum cultureCell adhesion assaysImproving survival of primary cells in cultureSourceRat tail tendonQuality ControlTested for ability to promote attachment and spreading of HT-1080 human fibrosarcoma cellsTested and found negative for bacteria and fungiCollagen I purity >90% by SDS-PAGEStorage4°C to 30°C under dry conditions.