Agarose is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marinealgae. As a gelling agent, agarose is used: 1.) to separate nucleic acids electrophoretically because…

L-Cysteine hydrochloride monohydrate (LCHCMH) is a water soluble salt of the non-essential amino acid, L-cysteine. Widely employed in the medicine industry Thermodynamic features of a solution of LCHCMH in water have been reported NMDA glutamatergic receptor antagonist L-Cysteine…

Saponin is "built" from quillaic acid, a sugar and possibly other substances. It is a Glycoside widely distributed in plants. 1% of saponin solution can hemolyze erythrocytes. Saponin solutions have been used in the following ways: concentrations of 0.005%-0.01% were used to…

Albumins are a group of acidic proteins which occur in the body fluids and tissues of mammals and in some plant seeds. Serum and plasma albumin is carbohydrate free and comprises 55-62% of the protein present. Bovine albumin is a single polypeptide chain consisting of approximately 583 to 595 amino…

Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first…

Deoxycholic Acid Sodium Salt Monohydrate is an anionic biological detergent for the solubilization of membrane-bound proteins. Anionic detergent; useful for extraction of membrane receptors and other plasma membrane proteins and for nuclei isolation. Deoxycholic acid has been used in a modified…

Benzalkonium Chloride is a quaternary ammonium salt. It is a cationic surface active agent and germicide. It is not effective against gram-negative bacteria or picornaviruses and it is not recommended for treatment of sanitizing critical or semicritical medical instruments. It has been reported…

Thiamine (Vitamin B1) is one of the essential vitamins and is required for carbohydrate metabolism. Thiamine hydrochloride is used as a food-additive to add a brothy/meaty flavor to gravies or soups. It is also used as food supplement. Used in fluorometric determination of mercury.

Methionine is one of the common sulfur-containing amino acids. Methionine is a common methyl-group donor to various substrates, such as creatine, epinephrine, ergosterol, and choline. L-Methionine is used as an essential amino acid for human development and therapeutically acts as an antidote…

Bis-Tris is a zwitterionic buffer; an amine buffer similar to Tris Hydrochloric acid that exhibits only a very small shift in dissociation constant with respect to temperature. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35…

β-D-Glucose is a main source of energy for living organisms. It is combined into glucosides, disaccharides, oligosaccharides, the polysaccharides (cellulose and starch), and glycogen. β-D-glucose may contain up to 10% of α-isomer.

* Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins * Used in the isolation of RNA * Solubilizes insoluble or denatured proteins such as inclusion bodies * Can be used for the recovery of periplasmic proteins

Albumins are a group of acidic proteins which occur in the body fluids and tissues of mammals and in some plant seeds. Serum and plasma albumin is carbohydrate-free and comprises 55-62% of the protein present. However, only about 40% of the total albumin in the body is in the circulating plasma at…

Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae. Allows recovery of active DNA after separation, with minimal inhibition of restriction and ligation enzymes Suitable for both analytical and preparative electrophoresis procedures Good…

β-D-Glucose is critical for production of proteins and the lipid metabolism. β-D-Glucose is a main source of energy for living organisms and is combined into glucosides, disaccharides, oligosaccharides, polysaccharides (cellulose and starch), and glycogen. D-Glucose, a naturally…

AlbumiNZ™ Low Fatty Acid BSA supports growth of stem cells and increases protein production from hybridoma cells. AlbumiNZ™ Low Fatty Acid BSA is chromatographically purified from New Zealand-sourced bovine plasma in an ISO quality system assuring complete traceability and…

L-(+)-Arabinose is the naturally occurring isomer and is a constituent of plant polysaccharides. L-(+)-Arabinose is used as a substrate to identify, differentiate and characterize pentose sugar isomerase(s). L-Arabinose is used in the bioproduction of L-ribose. L-Arabinose is the naturally…

L-histidine is an essential amino acid with a basic side chain, and is hydrophilic in character. It contains an imidazole group in the side chain. Histidine is biosynthesized from ATP, 5-phosphoribosyl-1-pyrophosphate (PRPP), and glutamine. In turn, histidine is degraded to glutamate by histidase,…

SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The…

MP offers this grade of albumin as a more highly purified product than our traditional True Cohn Fraction V Powder. Due to lower manufacturing costs, this grade is available at a substantial savings relative to our True Cohn Fraction V Powder. Albumin is general blocking reagent in ELISA, as a…

Cholesterol is the principal sterol of the higher animals, found in all body tissues. It is the main constituent of gallstones and all biological membranes. Approximately 25% of total brain lipid is cholesterol. Cholesterol, followed by the chemical suffix -ol for an alcohol, is an organic chemical…

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the…

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Bovine albumin is a single polypeptide chain consisting of approximately 583 to 595 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group. Albumin binds water, Ca2+, Na+, and K+. Due to a hydrophobic cleft, albumin binds fatty…

L-arginine is an amino acid. Necessary for the production of protein Helps rid the body of ammonia Stimulates the release of insulin Used to make nitric oxide Substrate of nitric oxide synthase, which is converted to citrulline and nitric oxide (NO). Induces insulin release by a…

Ammonium persulfate is a strong oxidizing agent. It is a radical initiator. Ammonium persulfate is a polymerization catalyst used with TEMED for polyacrylamide gel formation. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry. Other…

MOPS is used as a biological buffer. MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES; however, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4. A buffer using MOPS sodium salt can be…

L-Asparagine is used in cell culture media and is a component of MEM non-essential amino acids solution. L-Asparagine has been shown to enhance ornithine decarboxylase activity in cultured human colon adenocarcinoma Caco-2 cells and in cultured IEC-6 intestinal epithelial cells. Spore germination…

Cholesterol is the principal sterol of the higher animals, found in all body tissues. It is the main constituent of gallstones and all biological membranes. Approximately 25% of total brain lipid is cholesterol. Cholesterol is suitable for use in liposome preparations.

Guanidine hydrochloride consists of a network of guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds, it is a strong chaotropic agent. Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a free flowing powder…

Boric acid has a single boron; its salt forms complex equilibria in solution, with the ion B4O7-2 being the predominant species. Boric acid is used for weatherproofing wood and fireproofing fabrics; as a preservative; manufacturing of cements, crockery, porcelain, enamels, glass, borates,…

Tricine, a zwitterionic buffer, was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. The name tricine comes from tris and glycine from which it was derived. Tricine can be used in…