Bis-Tris is a zwitterionic buffer; an amine buffer similar to Tris Hydrochloric acid that exhibits only a very small shift in dissociation constant with respect to temperature. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.
Bis-Tris is suitable for fractionation of hemoglobin. A protocol for the agarose gel electrophoresis of glyoxylated RNA that uses Bis-Tris in the electrophoresis buffer has been described. The use of bis-Tris in polyacrylamide gels for the in-gel derivatization of proteins for cysteine-specific cleavages and MS analysis has been reported. A multiphasic buffer system for the SDS-PAGE analysis of proteins and peptides in the molecular weight range of 1-100 kDa that incorporates bis-Tris as a counterion in the stacking phase has been described. Bis-Tris buffer has been utilized in a study of λ cro repressor self-assembly and dimerization.
Grade: Cell culture reagent
Purity: ≥99%
Suitability: Suitable for cell culture
Key Applications: Used in electrophoresis buffer
Application Areas: Molecular Biology
Product Type: Biochemicals
Biochemical Category: Buffers
Melting Poin:t 101 - 106°C
Extinction Coefficient (E1%): EM (water): 259 nm = 10; 277 nm = 10 (Lit.)
Presentation: White Powder
Format: Powder
pH: 9 - 10 (1% aq soln); Useful pH Range: 5.8-7.2 (Lit.)
pKa: 6.5 (25°C)(Lit.)
Detection Method: FTIR
Solubility: Soluble in water (1 M - clear, colorless solution).
Sterilization of Solutions: For sterilization of aqueous solutions, MP Biomedicals recommends sterile filtration with a 0.2 µm filter; however, the product is typically stable for autoclaving.
Storage & Handling: Store at Room Temperature (15-30°C).