Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase.
Grade: Optical Density Grade
Key Applications: Strong denaturant
Application Areas: Molecular Biology
Product Type: Biochemicals
Biochemical: Category Chaotropic agent
Density: 1.345 g/cm3 at 20°C (Lit.)
Melting Point: 180 - 190°C
UV/Visible Absorbance: OD260 (6.0 M aq soln): ≤0.03
Presentation: White Crystalline Powder
Format: Crystalline Powder
pKa: 13.6 (Lit.)
Detection Method: FTIR
Solubility: Soluble in (6.0 M aq soln): Clear, Colorless Solution.
Storage & Handling: Store at Room Temperature (15-30°C). Store Desiccated.