Protein Loading Buffer (SDS-PAGE)-5X is a modified, 5X concentrated protein loading buffer containing bromophenol blue dye and SDS, but lacking DTT. It can be used for routine SDS-PAGE protein sample loading. Application Notes: Dilute to 1X with double distilled water or add to sample to…

Anti-globulin reagent (Coombs' serum) is prepared by immunizing rabbits with canine IgG, IgM and C3 purified from normal serum. The resulting whole antiserum is heat treated to inactivate complement and is adsorbed with normal pooled canine erythrocytes to remove nonspecific agglutinins. Canine…

Ultra-Fast Running Buffer for SDS-PAGE (Powder) is used in combination with traditional Tris-Glycine SDS-PAGE protein gels to achieve rapid electrophoresis. Compared with traditional running buffers, Ultra-Fast Running Buffer significantly shortens electrophoresis times (~ 20 minutes at 250 V) and…

This product is useful for structural and biological studies, as a reference standard, or as starting material for immunogen preparation. It is prepared using salt fractionation and/or chromatographic techniques. Purity: >98%

* Used to lyse living yeast cell walls to produce protoplast or spheroplast * Can be used for glucan hydrolysis Zymolyase-20T is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cells. This preparation is a…

Fibrinogen is a blood protein that is involved in clotting and is converted to fibrin by thrombin. Fibrinogen has an approximate molecular weight of 340 kDa. It consists of three non-identical pairs of disulfidebonded chains. The α-chain has an approximate molecular weight of 63.5 kDa, the…

The Quick Fluorescent Loading Buffer pre-stains a protein sample for SDS-PAGE. Protein samples are stained fluorescently during sample treatment prior to electrophoresis. Protein bands in an SDS-gel or on a membrane after transfer can be visualized and analyzed by a UV or LED transilluminator or a…

Aprotinin is a protein consisting of 58 amino acids, arranged in a single polypeptide chain that is crosslinked by three disulfide bridges. Aprotinin is found in bovine lymph nodes, lung, parotid gland, spleen, liver, pancreas, seminal vesicles, thyroid gland, kidney, mucous membranes of the…

Streptavidin is a protein produced by the bacterium Streptomyces avidinii. It has four binding sites for biotin, as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes or fluorochromes. However, avidin is highly basic (pI ~ 10.5),…

Apoferritin is the Protein shell of ferritin. Calibration of gel filtration columns and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis Acts as an anti-oxidant When added to cultured endothelial cells apoferritin is taken up in a dose-responsive manner and appears to…

Product reacts in an agglutination assay with anti-sheep red blood cells. The hematocrit is within the acceptable range. Red blood cells collected into Alsever's solution (Dextrose, sodium chloride, sodium citrate), washed with phosphate buffered saline and preserved with borate buffer,…

Fetuin is a protein containing 12 Cys residues, all involved in disulfide bridges. The composition of bovine fetuin (weight %) is polypeptide 74%, hexose 8.3%, hexosamines 5.5%, and sialic acid 8.7%. Fetuin bovine is a protein with the ability to bind to the insulin receptor and inhibit insulin…

Tris-Glycine Running Buffer is formulated for the separation of proteins in their denatured or native state on Tris-Glycine gels. This product is available in powder form. Each pack can prepare 500 mL of a 1X working solution, for a total of 5 L. Application Notes: Dilute the packet in 500…

β-Galactosidase cleaves terminal galactose residues, which are β -1-->14 linked to a monosaccharide, glycopeptide, or oligosaccharide. The enzyme requires Mg2+ for optimal activity. β-Galactosidase is useful for the structural investigation of carbohydrate, the determination of…

Fluorescent Protein Marker contains a total of 9 fluorescent proteins ranging from 14 kDa to 200 kDa. It is designed as a molecular weight standard for our Quick Fluorescent Protein Buffer, 08W00013 (SDS-PAGE)and 08W00014 (Native-PAGE), pre-stained protein samples, and other fluorescent protein…

Protein Loading Buffer (Native-PAGE)-5X is a modified, 5X concentrated protein loading buffer containing bromophenol blue dye. It can be used to prepare protein samples for non-denaturing gel electrophoresis using native PAGE gels. Application Notes: Dilute to 1X with double distilled water…

Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents. The agglutination activity of these highly specific carbohydrate-binding molecules is…

β-2-microglobulin (β2-M) is a polypeptide that associates with the heavy chain of class I major histocompatibility complex (MHC) antigens on the cell surface. The interaction is dynamic and plays a critical role in the stability of the MHC antigens and their ability to present peptide…

Pancreatin is a digestive enzyme produced by the pancreas and delivered to the small intestine for the hydrolysis of complex nutrients. Pancreatin contains enzymatic components including trypsin, amylase and lipase, ribonuclease, and protease, produced by the exocrine cells of the porcine pancreas.…

Tubulin, Purified from Bovine Brain, 1 mg. Purified bovine brain tubulin protein supplied in liquid form at 10 mg/mL in 80 mM PIPES buffer containing 1 mM EGTA, 1 mM GTP, pH 6.8 and 10% glycerol. It is a heterodimer composed of a- and ß- tubulin of 55 kDa (molecular weight ?110 kDa). Polymerization…

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the…

Bovine Transferrin is chromatographically purified from New Zealand-sourced bovine plasma in an ISO quality system assuring complete traceability and consistent high quality. Transferrin (HOLO) is an iron-rich product supplied as a heat-treated, lyophilized powder.

Each donor unit used in the preparation of the product was tested and found negative for Anti-HIV 1/2, HBsAg, Anti-HCV and HIV-1 Ag by FDA approved methods or CE approved methods. Used as an immunological challenge in studies of mechanisms of immunotolerance.

Lecithin is a purified, wax-like phosphatidylcholine of soybean origin. In addition to being the major structural component of cellular membranes, phosphatidylcholine serves as a reservoir for several lipid messengers, it is the source of the bioactive lipids lysophosphatidylcholine, phosphatidic…

Premixed liquid, 4X concentrate. Contains 250mM TRIS-HCl (pH6.8), 8% SDS, 40% glycerol, and 0.02% bromophenol blue. For protein sample preparation in the Laemmli SDS-PAGE system, without reducing the disulfide linkages.

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Glycoprotein which is a highly active thiol proteinase Non-specific cysteine protease Splits glycine esters but not lysine esters Protein digesting…

Product is the lyophilized powder of human hemoglobin and buffer salts. Human hemoglobin is purified from human red blood cells using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M…

The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 10 percent so that a minimum of 20 mg of mouse IgG is obtained upon reconstitution. Mouse IgG is purified from pooled mouse serum using multi-step…

Beef extract is a water soluble powder that is used in general purpose media. It is recommended for use in bacteriological examination of water, milk, and other materials, where uniform composition of media is important and for biochemical studies, particularly fermentation reactions because of its…

Casein is the name for a family of related phosphoproteins (αS1, αS2, β, κ). These proteins are commonly found in mammalian milk, making up 80% of the proteins in cow milk and between 20% and 45% of the proteins in human milk. Casein has a wide variety of uses, from being a…

Crude Phaseolus vulgaris-P lectin (PHA-P) from the Red Kidney Bean. Phytohemagglutinin PHA agglutinates the erythrocytes of all human blood groups as well as those of rabbit, dog, cat, mouse, sheep, and guinea pig. The optimal amount of PHA required for the agglutination of erythrocytes varies with…

Fibronectin are adhesive glycoproteins and are found both as cell surface proteins and in plasma. They are large glycoproteins found in cells and extracellular fluids. They are involved in a wide range of biological activities including cell adhesion to extracellular matrices, blood coagulation and…