Anti-globulin reagent (Coombs' serum) is prepared by immunizing rabbits with canine IgG, IgM and C3 purified from normal serum. The resulting whole antiserum is heat treated to inactivate complement and is adsorbed with normal pooled canine erythrocytes to remove nonspecific agglutinins. Canine anti-globulin reagent is presented in lyophilized form. One mL of reconstituted material is sufficient for 10 tests by the tube method or 50 when using the microtiter plate method.
Autoimmune hemolytic anemia (AIHA) is an autoimmune disease characterized by antibody coated red blood cells that either lyse in the presence of complement, or are subject to phagocytosis by the liver and spleen, resulting in a severe anemia.
Canine anti-globulin or Coombs' test is widely used to detect the presence of antibody in the diagnosis of autoimmune hemolytic anemia. Antibody alone or in the presence of complement injures the cell membrane permitting leakage of RBC material, allowing recognition by the reticuloendothelial cells, thereby leading to phagocytosis and destruction of RBC's. Immune mediated erythrocyte destruction is also seen in canine systemic lupus erythematosus some infections and in neoplastic disorders.
Reconstitute with deionized water. For catalog no. 64635-1 add 5.0 mL and for catalog no. 64635-2 add 1.0 mL water. Mix well to assure complete solubilization of the lyophilized material.
Key Applications: Autoimmune Hemolytic Anemia (AIHA) diagnosis
Application Areas: Immunoassays
Research Areas: Autoimmune Diseases
Product Type: Antibodies
Antibody Type: Primary Antibodies
Format: Lyophilized antiserum
NOTES: Anti-Canine Globulins- IgG, IgM, C3
Storage & Handling: Store at 2-8°C. After reconstitution, aliquot and store at -20°C, where it should be stable for at least 1 year. Avoid repeated freeze/thaw cycles. Discard if it becomes visibly contaminated.
Disease Screened: Autoimmune hemolytic anemia (AIHA)