Medium 199, originally described by Morgan and his colleagues (1950), is a completely defined nutritional source for cell culture. Their investigations demonstrated that cell growth could be measured in this medium. It has broad species applicability including the culturing of non-transformed cell…

Lipoprotein- cholesterol is a mixture of lipoprotein cholesterol, phospholipids and fatty acids. For use as a serum-free or serum-reduced media nutrient supplement, calibration of cholesterol values, and enzymatic or non-enzymatic cholesterol determinations.

Applications 7X cleaning solution was developed for use whenever a high degree of cleanliness is required. Test tubes, petri-dishes, flasks, cylinders, burettes, pipettes, slide cover glasses, hypodermic syringes and all other types of laboratory glassware and surgical instruments can be cleaned…

Polysorbate 20 is typically used as an emulsifying agent for the preparation of stable oil-in-water emulsions, particularly in pharmaceutical applications. It is also used in pre-extraction of membranes to remove peripheral proteins & as a blocking agent on nitrocellulose at a typical…

7X cleaning solution was developed for safe and effective cleaning of laboratory instruments and glassware. 7X-O-Matic is a powerful wetting and penetrating agent, which contains a non-ionic surfactant and a hydro-trope to complex phosphates. 7X-O-Matic was designed to clean under pressure, heat…

Tween 80 is a polyethylene sorbitol ester, non ionic surfactant and an emulsifier derived from polyethoxylated sorbitan and oleic acid. Tween 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture, growing of tubercule…

Starch is a carbohydrate consisting of a large number of glucose units joined by glycosidic bonds. It contains Approximately 75% amylopectin & 25% amylose. This polysaccharide is produced by all green plants as an energy store. It is the most common carbohydrate in the human diet and is…

Acetate plate sealer NS is easy to apply and remove from microplates. In addition, this microplate sealing tape is suitable for all microplates for both short- and long-term storage, providing a tight seal to minimize evaporation and condensation. * Used for sealing multi-well plates and…

Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.

BioRich 1 with 10 common anchorage dependent cell lines commonly show increased yields from 8% to 114% when compared to standard media in normal serum-containing systems. BioRich 1 also shows elevated growth of hybridoma lines at reduced serum concentrations when compared to normal base media.…

Oleic acid is a mono-unsaturated omega-9 fatty acid created by dehydrogenation of stearic acid. It is then further dehydrogenated and elongated to create members of the n-9 family of fatty acids. Oleic acid is used as emollient. Small amounts of oleic acid are used as an excipient in…

Rabbit Purified Lyophilized form of rabbit serum

Soybean oil (SBO) is a vegetable oil widely used as biodegradable lubricant. It may be transesterified to biodiesel using CaO as a solid base catalyst. It may also be transesterified to biodiesel using methanol and ethanol. Soybean Oil may be used as a crude source for triglycerides and other…

7X cleaning solution was developed for safe and effective cleaning of instruments, glassware, containers and more in laboratory and industrial settings. Several convenient package sizes are available. The product is sold in concentrated form to prepare the appropriate dilutions as needed. We…

Citric acid is a key metabolic intermediate. Citrate is the starting point of the tricarboxylic acid cycle. Its concentration also coordinates several other metabolic pathways. Citric acid can form complexes with various cations, particularly with iron and calcium. In animals, citric acid improves…

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals. Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to…

Chrome Azurol S is used as a reagent for determination of fluoride.

Stainless Steel Lysing Matrix Tubes are ideal for grinding, lysis and homogenization of your most resistant samples! Constructed from 308 SS, these tubes and grinding matrix are tough enough to stand up to the most demanding mechanical punishment that can cause traditional thermoplastic tubes to…

Lidocaine is the first amino amide–type local anesthetic. Lidocaine is used topically to relieve itching, burning and pain from skin inflammations, injected as a dental anesthetic or as a local anesthetic for minor surgery. A common local anesthetic and antiarrhythmic drug. Na+ channel…

MP Biomedicals' FastPrep-96 is a versatile high-speed homogenizer offering the ultimate in high-throughput sample preparation. Developed for resistant samples, the FastPrep-96 instrument uses a high-speed linear motion to thoroughly disrupt any tissues and cells in just seconds through the…

High-throughput adapter for the FastPrep-96 that holds 96 x 2 mL Lysing Matrix Tubes. The ergonomic design of the adapter ensures an easy loading of Lysing Matrix tubes that remain securely sealed during homogenization. Ideally suited for DNA and RNA isolation. Enzyme isolation and protein…

Titertek® premium-quality pipette tips allow your pipette to work the way it was designed— at peak performance and with the best possible accuracy and precision. To achieve this level of consistency, Titertek® tips are manufactured from only the best quality raw materials, and are…

The FastPrep-24 Classic instrument is a high-speed benchtop homogenizer offering the ultimate in speed and performance for the lysis of biological samples. Simultaneous homogenization of up to 24 samples takes place within 40 seconds. * Thorough and quick lysis of any tissues and cells *9…

To rehydrate the powdered medium, dissolve 29.5 grams in one liter of water. Dispense as desired. Sterilize in the autoclave for 15 minutes at 15 pounds pressure at 121°C (Catalog number 16821 is a pre-sterilized solution). For more rapid growth of pathogenic bacteria, dissolve 1 gram of agar…

β-Sitosterol is a common plant steroid that possesses anticholesteremic properties. β-Sitosterol has recently been shown to induce G2/M arrest, endoreduplication, and apoptosis through the Bcl-2 and PI3K/Akt signaling pathways.

CIRCLEGROW® is used for growth of E.coli and extends plasmid production past log phase. Molecular Biology Certified™ Obtain rapid growth of E.coli High yields of plasmid DNA At the end of log phase, cells growing in Circlegrow begin to utilize a proprietary second…

Clear Caps fit empty 2mL FastPrep Tubes and are used in Fastprep systems to hold sample for Homogenization. Available separately for those who wish to fill their own Lysing Matrix Tubes. Screw cap with o-ring.

Replacement teflon o-ring used in the cap of a SS Lysing Matrix tube to provide a leak-proof closure.

In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor…

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It has the same composition as Cleveland’s Trace Element I. To be added aseptically to media as a supplement. Prior to use of media, it may be necessary to adjust pH and sterile filter (optional, reduces contamination).

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance Provides cells with water and certain bulk inorganic ions…

Double decker adapter that holds 48 x 2 mL Lysing Matrix tubes. The ergonomic design of the adapter ensures easy loading of Lysing Matrix tubes that remain securely sealed during homogenization. It stands stable on the bench top and is commonly used as a tube rack. Ideally suited for…