Product is the lyophilized powder of human hemoglobin and buffer salts.
Human hemoglobin is purified from human red blood cells using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added.
All blood products should be treated as potentially infectious. Source material from which this product was derived was found negative when tested in accordance with current FDA required tests. No known test methods can offer assurance that products derived from human blood will not transmit infectious agents. The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 10 percent so that a minimum of 20 mg of human hemoglobin is obtained upon reconstitution.
Reconstitute product with 1 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Assay: The product is tested for purity at a minimum of 20 mg/mL using immunoelectrophoresis. The product is human hemoglobin; no trace of serum proteins is detected.
Concentration: Total Protein: 22.0 mg/mL, Volume: 1 mL
Application Areas: Immunoassays
Research Areas: Blood Proteins
Product Type: Reagents
NOTES: Purified antigen
Storage & Handling: Store unopened product at 2-8°C. Store reconstituted material no longer than a fewdays at 2-8°C unless sodium azide is added. For long-term storage, aliquot and freeze at -20°C.