XL1-Blue MRF’ and XL1-Blue MR competent cells allow representational cloning of methylated DNA. Transformation efficiency for XL1-Blue MR and XL-1 Blue MRF’ supercompetent cells of =1 x 10^9 transformants/µg Transformation efficiency for XL1-Blue MRF´ Kan…

qPCR Brilliant II SYBR MM with ROX Fast operation Available in a master mix format, including hotstart enzyme and reference dye;Low-abundance RNA Amplification and detection from total RNA, poly(A+), and synthetic RNA samples The Brilliant II qPCR SYBR master mix with ROX includes the…

Dramatically improve protein expression in E. coli by overcoming codon bias Suitable for expressing proteins from all species, including mammals;Ideal for difficult protein expression, especially when codon bias is a problem;Increases transformation efficiency with Hte phenotype;Derived from…

This special formulation of cloned Pfu DNA polymerase increases PCR product yields up to 19 kb. Robust performer with very low error rate (six times more accurate than Taq) Contains ArchaeMaxx factor for high-performance up to 19 kb for gDNA and 15 kb for vector DNA Available as a…

Improve the speed, efficiency and consistency of single-tube RT-PCR processes Reduced PCR cycling times and a one-tube, master mix format that is designed to reduce contamination, increase throughput and save valuable research time. High affinity for primer-template complexes, delivering…

Achieve consistent high specificity during qRT-PCR detection of miRNA in real time Achieve accurate real-time PCR quantification for miRNA. A high-specificity reagent system that delivers consistent, sensitive and reproducible results. The kit elongates miRNAs in a polyadenylation…

High quality single and multiple site-directed mutagenesis in less than 3 hours. For single site mutagenesis, one kit accommodates both short and long templates (4-14 kb). The kit delivers 80% mutagenesis efficiency for 1 site and 55% mutagenesis efficiency for 3 mutations…

qPCR Brilliant II SYBR MM These fast qPCR kits come in master mix format, including hotstart enzyme Low-abundance RNA;Amplification and detection from total RNA, poly(A+), and synthetic RNA samples The Brilliant II qPCR SYBR master mix includes the components necessary to carry out PCR…

Detects low copy number target and withstands PCR inhibitors without extensive purification. Robust amplification of genomic DNA targets up to 10 Kb High-specificity HotStart increases enzyme sensitivity Superior efficiency and yield with our patented ArchaeMaxx polymerase-enhancing…

Improves reliability and yield of conventional Taq-based PCR amplifications. Improves length, yield, and reliability of PCR;Enhances efficiency of template extension by Taq DNA polymerase Improves amplification of difficult targets Taq Extender PCR additive improves the reliability…

XL1-Blue Competent Cells are versatile for routine cloning and allow blue-white color screening. Designed to provide a host for optimal propagation of both plasmid and lambda phage vectors Available in a range of cloning efficiencies Available as economical subcloning-grade Competent…

Agilent PCR-grade dNTP and 10x DNA Polymerase Buffers can be used with any PCR application, including real-time quantitative PCR. Optimizes PCR performance Produces high-quality reaction products Withstands multiple freeze-thaw cycles without compromising efficiency Agilent…

InterPlay Mammalian TAP System provides proven purification of interacting proteins Recover intact interacting protein partners without protease digestion, as SBP and CBP elute easily from their affinity resins Exceptionally pure protein for downstream analysis;Includes all vectors,…

ABLE K Competent Cells for toxic clones reduce copy number 10-fold compared to the XL1-Blue strain. Reduces the copy number of ColE1-derived plasmids (such as pUC and pBluescript® plasmids) 10-fold Decreases level of cloned protein product to increase cell viability …

Enhance gene expression analysis with cell lysis and immediate stabilization. Save time and money by purifying only the most relevant samples after efficiently screening. RNA is stabilized for at least six months to allow sample archival. Multiple product formats offer maximum choice…

Improved detection sensitivity, plus reproducible quantification even at low target concentrations. Greater flexibility for use with numerous templates and targets Developed on Agilent Mx qRT-PCR Systems Passive reference dye to maximize PCR probe performance on different instrument…

Proteinase K is ideal for protein digestion in biological samples and preparing chromosomal DNA. For general digestion of protein in biological samples Lyophilized powder with specific activity of 30units/mg at 37ºC;Guaranteed stability for 6 months when stored correctly …

Test your qPCR system for errors with the AriaMx SYBR Qualification Plate Kit. Contains one 96-well plate Each well contains 20 OL of qPCR reagents The AriaMx SYBR Qualification Plate Kit is for use in a qualification test on the AriaMx Real-Time PCR System. Running a qualification test is one…

Brilliant III Ultra-Fast SYBR qRT-PCR Master Mixes utilize a novel faster-activating hot start method Provides specificity by utilizing a novel faster-activating hot start method that minimizes formation of primer-dimers and other off-target reactions Pre-blended formulations are compatible…

A 50-kDa protein that specifically inhibits common eukaryotic RNases Only 25 to 40 U is required for a standard transcription reaction. Monitored for endogenous exonuclease and endonuclease activity. Determined to be free of contaminating RNases by incubating RNA transcripts. …

Brilliant III qPCR probe master mix with ROX is one of our fastest, high-performance, ultrasensitive, next generation qPCR master mix reagents. Fast kit with speeds at 40 min per 40 cycles Comes in master mix format, including hotstart enzyme and reference dye Low-abundance RNA, down to…

Competent cells used to prepare plasmid or phagemid cells for more accurate analysis High quality competent cells for preparing methylation-free DNA. The SCS110 strain lacks both dam and dcm activity, and DNA propagated in this strain can be digested by methylation-sensitive enzymes such…

Agilent’s AffinityScript Multiple Temperature cDNA synthesis kit delivers high yields of cDNA using a broad range of temperatures for your application. Robust cDNA synthesis across a broad range of temperatures Recommended up to 60 °C for RNA with increased 2 °C…

Provides the highest efficiency of conversion of RNA to cDNA, fully optimized for qPCR applications. Optimized for real-time quantitative PCR (qPCR) application;Produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg total RNA;Creates up to 12 kb cDNA in 25 minutes or…

Cloned Pfu DNA Polymerase for High Fidelity and Difficult, GC-Rich PCR. Highly thermostable Pfu DNA polymerase possessing 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors (1 error per 770,000 bp). Reformulated…

PfuUltra High Fidelity DNA Polymerase AD features a reformulated buffer system and Hotstart mechanism for increased economy with the same high fidelity with long targets as the original PfuUltra Hotstart DNA polymerase. Robust PCR product yields with a wide range of targets up to 19 kb of…

High quality human reference RNA from multiple cell lines; pooled and treated to ensure consistency. Enables greater consistency than RNA references derived from tissue sources. Provides the maximum representation of low, medium, and high abundant gene transcripts. Manufactured at…

Get easy detection of expression with humanized hrGFP, qualitatively assess transfection efficiency Qualitatively assess the transfection efficiency of the producer cell line Determine the viral titer using fluorescence microscopy or by fluorescence activated cell sorting (FACS) Use…

High quality plasmid vectors for molecular cloning and other DNA sequencing research applications A high copy number ColE1-based phagemid. A large and versatile polylinker in two orientations f1 origin available in either (+) or (-) orientation.;T3 and T7 promoters for in vitro…

Our cDNA Synthesis kits are designed for the highest efficiency conversion of RNA to high quality cDNA. Our kit uses a fast, easy-to-use 15-minute cDNA synthesis step that allows you to generate cDNA up to 12 kb in less than 25 minutes. Achieve a robust cDNA yield with even GC-rich sequences. …

The AD-293 cell line simplifies the process of producing recombinant adenovirus particles. For use with Agilent InterPlay Adenoviral TAP System and AdEasy vectors Improved adherence to tissue culture dishes over standard HEK293 cells Simplifies the process of producing recombinant…

The high-purity DpnI restriction enzyme is ideal for cloning and Southern blot analysis. High purity enzyme preparation;Includes 10X universal reaction buffer The high-purity DpnI restriction enzyme is ideal for cloning and Southern blot analysis, and used for site-directed mutagenesis.…