DNA Gel Loading Solution, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. …

Minimize contamination with Endotoxin-Free Water. Eliminate the risk of toxic activity produced by bacteria that can interfere with cell structure and function. Mycoplasma tested DNase, RNase and protease tested Endotoxin < 0.005 EU/mL Applications Ideal for preparing solutions…

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Applications DPBS can be used to provide a buffer system to…

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …

PCR Water is useful for PCR Assays (i.e rRT-PCR, etc.). DNase, RNase and Protease tested. Free of contaminants of human, mouse and rat DNA. Applications PCR Qualified Water is functionally tested in a PCR reaction. PCR Qualified Water is highly purified water and it is reverse osmosis…

MOPS Buffer is used at 1X for the electrophoresis of RNA as the running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels. MOPS buffer is also used for Northern Blots -the hybridization of total RNA or mRNA to a membrane. Liquid, 10X concentration Filtered…

Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride and provide convenient and highly purified solutions. Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its…

This is a special formulation of loading solution for protein gel electrophoresis. Special formulation Applications SDS-PAGE Western Blotting Analysis of DNA samples with high amounts of DNA binding proteins Kinetic experiments DNA agarose gel analysis after DNA restriction digestions,…

SDS Protein Gel Loading Solution, 2X is formulated at the traditional 2X stocks, for standard volume of sample to be loaded per well. Simply load the gel sample wells and visualize progress of the electrophoresis run by monitoring the location of the blue dye front. Lane Marker Sample Buffers…

EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and…

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …

TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. DNase, RNase and Protease tested. Applications TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can…

TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. Filtered through a 0.22 µm…

TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. Applications EDTA is a chelator of divalent cations,…

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. Without EDTA Applications Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223…

Amphotericin B is used to prevent the contamination of cell cultures by yeast and multicellular fungi. 100X concentration. Just dilute 1:100 to get the final working concentration of 2.50 µg/mL Antifungal agent Amphotericin B prevents the growth of fungi by causing an increase…

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. Applications ACK Lysing Buffer is used to lyse red blood cells in samples, such as EDTA-treated…

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …

Protein Running Buffer, 10X is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE), and is the running buffer for TRIS-glycine gel electrophoresis. Contains 1% SDS Filtered through a 0.22 µm…

Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride and provide convenient and highly purified solutions. Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its…

Nuclease and Endotoxin-Free, Sterile DEPC-treated water is certified nuclease-free and supplied in four bottles containing 1 L each to ensure sterility and inactivation of DEPC. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity, protease. …

Sodium Bicarbonate (NaHCO3) is typically used in cell culture media as a buffer. Endotoxin and Mycoplasma Tested Applications Although NaHCO3 does not have the buffering capacity of HEPES, it is occasionally preferred over HEPES because of its lower toxicity and nutritional value to cells…

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. Ready-to-use solution Applications Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis and…

Formulated as 5X rather than the traditional 2X stocks, SDS Protein Gel Loading Solution 5X enables a larger volume of sample to be loaded per well. 5X concentrated protein gel loading buffer Applications Simply load the gel sample wells and visualize progress of the electrophoresis run by…

RNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989). The purpose of this reagent is to insure that the RNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. Contains two dyes, bromphenol blue…

Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications …

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. Applications Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223 amino acid residue…

MEM NEAA contains the same non-essential amino acids found in the standard MEM, at a strength of 100. MEM NEAA 100X can be used to enhance cell growth and prolong the viability of cells in culture. 100X of Non-essential amino acids MEM NEAA 100X is commonly used as a supplement to minimal…

L-glutamine is a cell culture supplement, and an essential amnio acid. L-glutamine is required in cell culture for cellular growth. 100X concentration Applications L-glutamine is used in cell culture as an energy source in addition to carbohydrates (i.e. glucose, sucrose, maltose, etc.).…

Proprietary Sheath Fluid Prepared using water deionized by reverse osmosis 1µm sterile filtered Gamma irradiated to a minimum of 25kGy and maximum of 50kGy Applications Designed specifically for flow cytometry applications.

Tris HCl 2M pH 6.8 is useful with SDS-PAGE and Electrophoresis. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).Tris…

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. Applications Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223 amino acid residue…