PCR Reagents

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  • ROCHE Expand™ Long Template PCR System

    MilliporeSigma

    Synonyms: long range pcr; long template pcr Storage: −20°C UNSPSC Code: 12352200 Components: Expand Long Template Enzyme Mix; Expand Long Template Buffer 1, with 17.5 mM MgCl2 10x concentrated; Expand Long Template Buffer 2, with 27.5 mM MgCl2

  • KOD DNA Polymerase

    MilliporeSigma

    KOD DNA Polymerase* (formerly KOD HiFi DNA Polymerase) is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase (Nishioka 2001). KOD is a high fidelity thermostable polymerase that amplifies target DNA up to 6 kbp with superior accuracy and yield (Takagi 1997). The enzyme’s 3'?5'…

  • ROCHE FastStart TaqMan® Probe Master

    MilliporeSigma

    UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport Features and Benefits: •  Increase qPCR sensitivity and specificity: Produce lower cycle threshold (C t ) values with the hot start enzyme FastStart Taq DNA Polymerase and highest purity nucleotides included in the…

  • FastStart™ High Fidelity PCR System

    MilliporeSigma

    The FastStart High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein, which is also chemically modified. This protein mediates proofreading activity, but carries no polymerase activity. Both proteins are inactive below +75°C and are activated…

  • ROCHE High Pure PCR Cleanup Micro Kit

    MilliporeSigma

    UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport Analysis Note: A 341 bp PCR fragment of the tPA gene was amplified according to a standard block cycler protocol. The resulting reaction mixes were pooled and purified with the High Pure PCR Cleanup Micro Kit. Different amounts…

  • ROCHE First Strand cDNA Synthesis Kit for RT-PCR (AMV)

    MilliporeSigma

    Specific Activity: >50 units/μg protein Storage: −20°C UNSPSC Code: 41105903 Components: Reverse Transcriptase AMV; PCR Nucleotide Mix, pH 8.5 10 mM each; Reaction Buffer; MgCl2 Stock Solution 25 mM; Gelatin 0.05% (w/v); Oligo-p(dT)15 , 0.02…

  • KOD Hot Start DNA Polymerase

    MilliporeSigma

    KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'?5' exonuclease activities at ambient temperatures (Mizuguchi 1999). KOD Hot Start combines the high fidelity, fast extension speed, and outstanding…

  • NOVATAQ PCR Master Mix

    MilliporeSigma

    The NovaTaq™ PCR Master Mix is a ready-to-use 2X mixture of NovaTaq DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer without MgCl2. The Master Mix simplifies the assembly of PCR reactions and offers advantages of time savings, consistency, and minimal risk of contamination. Simply…

  • KOD XL DNA Polymerase

    MilliporeSigma

    KOD XL DNA Polymerase* is an optimized blend of KOD DNA Polymerase and a mutant form of KOD that is deficient in 3'?5' exonuclease activity (Nishioka 2002). This enzyme mixture is designed for reliable amplification of long, complex targets with robust yield and high accuracy. It can also be used…

  • ROCHE Water, PCR Grade

    MilliporeSigma

    Storage: −20°C UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport Application: This product is specially tested and qualified for use in all PCR or RT-PCR. It can be used in conjunction with all end-point or real-time PCR assays or whenever highest quality…

  • Extract-N-Amp(TM) Tissue PCR Kit, sufficient for 100 extractions, sufficient for…

    MilliporeSigma

    Storage: -20C UNSPSC Code: 12352200 Features and Benefits: . Perfect for quick genomic DNA isolation for genotyping. Single-step extraction of genomic DNA for PCR in 15 minutes. PCR ReadyMix is specially formulated for amplification directly from extract. Hot Start…

  • NOVATAQ Hot Start Master Mix Kit

    MilliporeSigma

    NovaTaq™ Hot Start Master Mix provides a ready-to-use 2X mixture of NovaTaq Hot Start DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer with MgCl2. The Master Mix simplifies the set-up for PCR and offers advantages of time savings, consistency, and minimal risk of contamination. In…

  • NOVATAQ DNA Polymerase

    MilliporeSigma

    NovaTaq™ DNA Polymerase* is a premium quality, recombinant form of Thermus aquaticus DNA polymerase that is licensed for PCR. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest purity and reproducible perfomance, each preparation is extensively tested…

  • ROCHE Deoxynucleoside Triphosphate Set, PCR Grade, sodium salt

    MilliporeSigma

    Synonyms: datp; dctp; dgtp; dttp; set of datp, dctp, dgtp, and dttp, pcr grade Purity: <0.9% (dNDP); 99% (dNTP) Storage: −20°C UNSPSC Code: 12352200 Components: dATP, PCR Grade, sodium salt 100 mM; dCTP, PCR Grade, sodium salt 100 mM; dGTP, PCR Grade, sodium salt 100…

  • NOVATAQ Hot Start DNA Polymerase

    MilliporeSigma

    NovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature. The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as…

  • NTI-FLAG® M2 Affinity Gel purified immunoglobulin, buffered aqueous glycerol…

    MilliporeSigma

    Synonym: ANTI-FLAG® M2 Affinity Agarose Gel, Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2 antibody produced in mouse antibody form: purified immunoglobulin form: buffered aqueous glycerol solution isotype: IgG1 capacity: >0.6 mg/mL, resin binding capacity (FLAG-BAP)…

  • ROCHE Mycoplasma PCR ELISA

    MilliporeSigma

    Storage: −20°C UNSPSC Code: 12352200 Components: Lysis Reagent; Neutralization Reagent; PCR Mix ready-to-use; Denaturation Reagent; Hybridization Buffer; Biotinylated Capture Probe; Washing Buffer 10x concentrated; Anti-Digoxigenin-POD antibody; Conjugate Dilution Buffer; TMB…

  • KOD Xtreme Hot Start DNA Polymerase

    MilliporeSigma

    The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR system for the amplification of long or GC-rich DNA templates. The unique formulation enables you to amplify directly from minimally processed samples; allowing highly efficient crude sample PCR target amplification. The system…

  • KOD Hot Start Master Mix

    MilliporeSigma

    KOD Hot Start Master Mix* is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4. The Master Mix simplifies PCR set-up, offering time savings,…

  • ROCHE TeloTAGGG™ Telomerase PCR ELISAPLUS

    MilliporeSigma

    Storage: −20°C UNSPSC Code: 41116133 Components: Lysis Reagent; Reaction Mixture 2x concentrated; Internal Standard (IS); Control Template, low; Control Template, high; Water, nuclease-free; Denaturing Reagent; Hybridization Buffer T; Hybridization Buffer IS; Washing Buffer 10x…

  • ROCHE PCR DIG Labeling MixPLUS

    MilliporeSigma

    Storage: −20°C UNSPSC Code: 41106000 RIDADR: NONH for all modes of transport Application: For direct labeling of amplification products with digoxigenin-dUTP in PCR reactions and for carryover prevention.The PCR DIG Labeling Mix plus is 10x concentrated. For each reaction,…

  • ROCHE dCTP, PCR Grade, sodium salt

    MilliporeSigma

    Synonyms: 2′-Deoxycytidine 5′-triphosphate disodium salt; dCTP-Na 2 Empirical Formula (Hill Notation): C 9 H 14 N 3 Na 2 O 13 P 3 Formula Weight: 511.12 Linear Formula: C 9 H 14 N 3 Na 2 O 13 P 3 MDL Number: MFCD00084683 Purity: <0.9% (dNDP); 99% (dNTP) Storage: …

  • ROCHE AptaTaq Fast PCR Master

    MilliporeSigma

    Storage: 2-8°C UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport Features and Benefits: Save time • 4 minutes processing time only. • Immediate enzyme activation. Amplify with Confidence • One-for-all protocol. • Room…

  • ROCHE Titan One Tube RT-PCR Kit

    MilliporeSigma

    Storage: −20°C UNSPSC Code: 41106300 Components: Titan Enzyme Mix; dNTP Mix, mixture of dATP, dCTP, dGTP, and dTTP solution 10 mM; RNase Inhibitor 5 U/μl; Human Control RNA 2 pg/μl; Control Primer Mix 20 μM; Water, PCR Grade; RT-PCR Reaction Buffer 5x concentrated;…

  • ROCHE PCR ELISA, DIG-Labeling

    MilliporeSigma

    Storage: −20°C UNSPSC Code: 41116133 Components: PCR DIG Labeling Mix, containing 2 mM dATP, dCTP, dGTP, 1.9 mM dTTP, and 0.1 mM Digoxigenin-dUTP; PCR Reaction Buffer, without MgCl2, 100 mM Tris/HCl, 500 mM KCl, pH 8.3 (20 °C) 10x concentrated; MgCl2

  • ROCHE PCR Master

    MilliporeSigma

    Storage: −20°C UNSPSC Code: 41106300 Components: PCR Master Mix (10 vials) 2x concentrated; Water, PCR Grade (5 vials) RIDADR: NONH for all modes of transport Features and Benefits: •  Reliable reproducible results: High lot-to-lot consistency.…

  • ROCHE Expand™ High FidelityPLUS PCR System

    MilliporeSigma

    Synonyms: high fidelity pcr plus system, expand Storage: −20°C UNSPSC Code: 12352200 Components: Expand High Fidelity PLUS Enzyme Blend 5 U/μl; Expand High Fidelity PLUS Reaction Buffer, with 7.5 mM MgCl2 5x concentrated; Expand High Fidelity PLUS …

  • ROCHE Proteinase K, recombinant, PCR Grade, Solution from Pichia pastoris

    MilliporeSigma

    Specific Activity: ~2.5 units/mg protein (Approximately 2.5 U/mg (Chromozym assay); ≥30 U/mg hemoglobin assay. Refer to the Certificate of Analysis for specific values for the present lot.) Storage: 2-8°C UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport …

  • ROCHE Strip PCR Tubes and Caps

    MilliporeSigma

    Storage: 20-25°C UNSPSC Code: 41121702 RIDADR: NONH for all modes of transport Application: Strip PCR Tubes and Caps are designed for use in PCR thermal cyclers, and provide optimal and rapid heat transfer. These tubes are ideally suited for use with the Expand PCR System, or with…

  • ROCHE FastStart Universal Probe Master (Rox)

    MilliporeSigma

    UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport Features and Benefits: •  Increase qPCR sensitivity and specificity. Produce lower cycle threshold (Ct) values. •  Use the master mix with any probe-based assay. Achieve sensitive, specific results in…

  • ROCHE FastStart™ PCR Master

    MilliporeSigma

    Storage: −20°C UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport Features and Benefits: The FastStart Master has modified recombinant FastStart Taq DNA Polymerase, inactive at temperatures below +75°C. The kit also contains an optimized PCR buffer and…

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