• PRODUCT AVAILABILITY: Did you know you can view a product's availability right on the product page? Simply enter the quantity you want to purchase and the current availability will appear below the item.

  • Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

MilliporeSigma

KOD Xtreme Hot Start DNA Polymerase

Not yet rated

The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR system for the amplification of long or GC-rich DNA templates. The unique formulation enables you to amplify directly from minimally processed samples; allowing highly efficient crude sample PCR target amplification. The system includes an ultra high fidelity KOD DNA polymerase complexed with two monoclonal antibodies to permit hot start thermocycling, along with specially formulated 2X buffer. KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively. KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content. Each kit provides 200 U KOD Xtreme Hot Start DNA Polymerase, an optimized buffer and dNTPs sufficient for 200 amplification reactions. The polymerase produces blunt-ended DNA products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits.

*Manufactured by Toyobo and distributed by EMD. Not available from Merck in Japan.

Features and Benefits:

  • Optimized for the highest PCR success rate, even with the most difficult targets
  • 10X higher fidelity than Taq blends
  • Efficiently amplifies up to 90% GC-content DNA templates
  • Amplifies genomic targets up to 24 kbp
  • Amplifies phage/Lambda targets up to 40 kbp
  • Eliminates mispriming and primer-dimer formation
  • Convenient ambient-temperature setup compatible with automation

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl2, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA.

Components:

  • 200 U KOD Xtreme™ Hot Start DNA Polymerase
  • 1.7 ml 2X Xtreme Buffer
  • 1 ml dNTPs (2 mM each)
Storage: -20°C 
Ship:Shipped with Blue Ice or with Dry Ice - Irritant 
S Phrase  S:26-36 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. 
R Phrase  R:36/37/38 - Irritating to eyes, respiratory system and skin.

Please Enter Your Order Info

Filter by:

  • Clear Filters
Product Detail
Thomas No.
C974W89
Mfr. No.
71975-3
Description
KOD Xtreme Hot Start DNA Polymerase 200u

list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

Write A Review