Synonyms: 2′-Deoxycytidine 5′-triphosphate disodium salt; dCTP-Na2
Empirical Formula (Hill Notation): C9H14N3Na2O13P3
Formula Weight: 511.12
Linear Formula: C9H14N3Na2O13P3
MDL Number: MFCD00084683
Purity: <0.9% (dNDP); 99% (dNTP)
Storage: −20°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.
General description:
dCTP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
Other Notes:
For general laboratory use.
Quality:
Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.
Physical form:
solution of dCTP in water (pH 8.3).
Preparation Note:
Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).