PCR Reagents

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  • SensiFAST™ SYBR® No-ROX One-Step Kit

    Bioline

    Sensitive, reproducible detection of even the most challenging RNA targets, under fast thermal cycling conditions. Product Highlights Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets Reproducible –…

  • MyTaq™ HS Red Mix

    Bioline

    A convenient mastermix containing a new generation of hot-start polymerase that delivers improved yield, sensitivity, speed and robustness when amplifying targets from any template. Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby…

  • Choice-Taq™ DNA Polymerase, 5000u

    Denville

    Concentration: 5u/µl Recombinant Taq DNA polymerase Supplied with a 10x NH4 Reaction buffer containing 1.5mM MgCI2 (final concentration)

  • JetSeq™ Library Quantification Hi-ROX Kit

    Bioline

    Accurate, reliable and reproducible qPCR-based method for quantifying DNA libraries prior to NGS on Illumina platforms. Accurate - qPCR-based assay for quantification of only adapter-ligated library molecules, thereby enabling optimal flow cell loading for maximum data yield and quality. …

  • MyTaq™ Extract-PCR Kit

    Bioline

    Many DNA extraction methods can be laborious and time consuming or involve the use of hazardous chemicals. MyTaq™ Extract-PCR Kit offers a rapid, easy and safer alternative for the extraction and amplification of DNA from a variety of tissue types. MyTaq Extract-PCR Kit is particularly suited…

  • PCR Nucleotide Sets

    MP Biomedicals

    Set of four dNTPs Separate vials of four dNTPs Concentrated neutralized solutions dNTPs Set 1 is composed of four separate vials of highly purified PCR grade, sodium salts of dATP, dCTP, dGTP and dTTP. The four dNTPs are in solution at a 100 mM concentration. This format allows the…

  • BIOTAQ™ DNA Polymerase

    Bioline

    Formally BIOLASE BIOTAQ is a highly purified, thermostable DNA polymerase offering high yield over a wide range of PCR templates and a good choice for routine PCR assays. BIOTAQ is a robust preparation and delivers high yields with minimal background. BIOTAQ possesses 5´-3´…

  • Choice-Taq™ DNA Polymerase, 1000u

    Denville

    Concentration: 5u/µl Recombinant Taq DNA polymerase Supplied with a 10x NH4 Reaction buffer containing 1.5mM MgCI2 (final concentration)

  • Custom Taq Polymerase, 100,000U W/200mL 10x BUFF & 200mL MgCl2 SOL

    Denville

    Custom Taq Polymerase, 100,000U W/200mL 10x BUFF & 200mL MgCl2 SOL

  • Accuris™ High Fidelity DNA Polymerase

    Accuris Instruments

    50X higher fidelity than Taq DNA polymerase Works with crude DNA sample Ideal for cloning, mutagenesis and microarrays Optimized buffer system with unique PCR enhancers For applications requiring highly accurate amplification, choose Accuris High Fidelity DNA polymerase. Modified for…

  • SensiFAST™ SYBR® Lo-ROX Kit

    Bioline

    Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions. Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Specific –…

  • ACTaq™ Blue Long DNA Polymerase

    ACTGene, Inc.

    ACTaq™ Long DNA Polymerase is a specialty taq for high processivity and fidelity at extension amplification speed fo 1000 bases per minute and optimized b etween 65-75oC, when using dNTP concentration at 100-500µM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.2.

  • Choice Taq Mastermix™, 1.5mM MgCl2, 400 reactions

    Denville

    Choice Taq Mastermix is a 2X reaction mixture. It is ready-to-go and it only requires addition of template, primers, and water. Choice Taq Mastermix diminishes the amount of time needed to set up reactions, thereby reducing the risk of contamination. Greater reproducibility is also ensured by…

  • SensiMix™ SYBR® & Fluorescein Kit

    Bioline

    Outstanding assay reproducibility and sensitivity for both DNA and cDNA templates, under standard conditions. Product Highlights Sensitive - reproducible detection of low-copy number templates Reproducible – consistent results between technical replicates for increased…

  • MyTaq™ DNA Polymerase

    Bioline

    MyTaq™ DNA Polymerase is recommended for all standard PCR applications. The MyTaq DNA Polymerase and MyTaq Reaction Buffer in this product, are a unique combination of next-generation polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR…

  • dNTP Sets

    Bioline

    The ready-to-use molecular grade dNTP Sets consist of four separate 100 mM solutions of dATP, dCTP, dGTP and dTTP respectively. Product Highlights High quality - >99% purity determined by HPLC, ideal for use in PCR reactions Long shelf-life - no need for aliquoting …

  • Hot-Start™ Taq Blue Mastermix™, 1.5mM MgCI2, 100 reactions

    Denville

    Hot-Start Taq Mastermix™ is the heat activated version of Denville's 2X, ready to go reaction mixture. It is inactive at room temperature and only requires addition of template, primers, and water. A 5 minute pre-heat step at 95°C is required to successfully carry out primer…

  • Denville rAmp™ High Fidelity DNA Polymerase

    Denville

    Highly accurate - 50X higher fidelity than Taq polymerase Suitable for a wide range of targets - crude samples as well as those rich in GC or AT sequences Ideal choice for cloning Exhibits both 5’ -3’ polymerase activity and 3’-5’ exonuclease activity Provided…

  • Choice-Taq Blue™ DNA Polymerase, 1u/µl, 5000u

    Denville

    Choice-Taq Blue™ DNA Polymerase is supplied with an inert blue dye that allows the end user a method to confirm addition of the enzyme to the reaction. The dye further allows the user to directly add a post-reaction sample directly into an agarose gel, eliminating the need for a separate…

  • Hot-Start Taq Mastermix™, 1.5mM MgCl2, 100 reactions

    Denville

    Hot-Start Taq Mastermix™ is the heat activated version of Denville's 2X, ready to go reaction mixture. It is inactive at room temperature and only requires addition of template, primers, and water. A 5 minute pre-heat step at 95°C is required to successfully carry out primer…

  • SensiFAST™ Probe Lo-ROX One-Step Kit

    Bioline

    Sensitive, reproducible detection of even the most challenging RNA targets, under fast thermal cycling conditions. Product Highlights Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets Reproducible –…

  • Hot-Start Taq™ Blue DNA Polymerase, 1u/µl, Pack: 250u

    Denville

    Inactive at room temperature Increased specificity This is the Hot-Start Blue version of Choice-Taq™Blue DNA Polymerase that requires a pre-activation step at 95°C for 5 minutes in order to achieve a fully functional polymerase enzyme. The inactivity of the enzyme at room…

  • MyFi™ Mix

    Bioline

    Unique blend of highly-efficient MyTaq HS DNA Polymerase and a proprietary proofreading enzyme that combine to give increased target affinity for use with challenging templates and inhibitor-rich samples. Robust - enzyme blend and buffering system promotes reliable amplification of the most…

  • KOD Hot Start DNA Polymerase

    MilliporeSigma

    KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'?5' exonuclease activities at ambient temperatures (Mizuguchi 1999). KOD Hot Start combines the high fidelity, fast extension speed, and outstanding…

  • MangoTaq™ DNA Polymerase

    Bioline

    MangoTaq™ DNA Polymerase is a formulation of Taq DNA Polymerase that offers high-yield across a wide range of DNA concentrations. MangoTaq DNA Polymerase possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang, resulting in PCR product suitable for effective…

  • Fast Probe Master Mix

    Biotium

    Fast Probe Master Mix is formulated for conducting real-time PCR using an oligo-based probe, such as a TaqMan® probe, a MGB probe or an AllGlo™ probe. The master mix contains buffer, dNTPs, MgCl2 and our superior hotstart enzyme Cheetah™ Taq.

  • ACTaq™ Blue Mastermix 2x

    ACTGene, Inc.

    ACTaq™ Blue / Red Mastermix is complete “ready-to-go” 2X reaction mix which only requires consumer researcher to add water, template and primers. ACTaq™ Blue / Red Mastermix differs from ACTaq™ Mastermix such that it contains blue / red dye added to the enzyme used for traccking adn loading dye…

  • dNTP Mix

    Bioline

    Ready-to-use molecular grade dNTP Mixes containing dATP, dCTP, dGTP and dTTP, designed to save hands-on time for researchers and minimize the possibility of contamination. Product Highlights High quality - >99% purity determined by HPLC, ideal for use in PCR reactions Long…

  • ACTaq™ Taq DNA Polymerase

    ACTGene, Inc.

    p> ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular weight of 94KDa. For use in DNA fragment extension amplification reactions with DNA having dA overhang on 3’ ends; at extension amplification speed of 1200 bases per minute and optimized between 65-75oC. Optimized for best…

  • Accuris™ 1 Hour Mammalian Genotyping Kit

    Accuris Instruments

    DNA extraction and amplification in 1 hour Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues Single tube - no organic solvents or clean up procedures Enhanced Accuris Hot Start Polymerase included Traditionally, mammalian genotyping protocols…

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