Storage: −20°C
UNSPSC Code: 41105324
RIDADR: NONH for all modes of transport
Application:
• Actin RNA Probe, DIG-labeled, is specifically useful for evaluating the quality and quantity of various RNA species and can be used for: In situ hybridization (for example, as a control in mRNA detection)
• The quality control in the construction of cDNA libraries
• Northern blot analysis to evaluate RNA from various human cell lines and tissue samples
General description:
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Components:
Actin RNA Probe, DIG-labeled, is supplied in autoclaved, DEPC-treated water.
Preparation Note:
Working solution: Dilute Sample• Remove the desired number of isotyping strips from the canister. Remove the caps from an equal number of development tubes.
The tubes may be labeled with a pencil or felt-tipped lab marker for easy identification.
• Dilute a sample containing the mouse monoclonal antibody in 1% BSA/ phosphate-buffered saline (PBS), pH 7.2 to 7.6.
Culture supernatant samples should be diluted 1:10 to 1:100. Ascites samples should be diluted 1:20,000. These are recommended dilutions and may vary depending on the concentration of antibody in your sample. In our experience, a monoclonal antibody
concentration of 0.1 to 1 g/ml of diluted sample gives the best results. 150 ml of this diluted sample will be added to the development tube.
Storage conditions (working solution): During assay, the preparation should be maintained at 0 °C.
Sequence:
Agarose gel electrophoresis under denaturing conditions and subsequent northern blot analysis reveal a defined band of 588 bases.