UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Features and Benefits:
• Increase qPCR sensitivity and specificity:
Produce lower cycle threshold (Ct) values with the hot start enzyme FastStart Taq DNA Polymerase and highest purity nucleotides included in the master mix.
• Use it with any probe-based assay:
Achieve sensitive, specific results in assays with the Universal ProbeLibrary Probes or any other hydrolysis probe.
• Amplify and detect a broad range of DNA or cDNA targets:
Amplify fragments up to 500 bp long, including those that are GC- or AT-rich.
• Use with any real-time qPCR instrument other than the LightCycler® Instruments:
Choose from two formulations - one that contains the ROX reference dye and one without ROX.
• Visualize amplification products on agarose gels.
• Use robotic pipetting stations to set up qPCR reactions:
Benefit from a master mix that is stable at room temperature during extended reaction setup times.
• Prevent carryover contamination:
The mix contains dUTP, so that it may be used with Uracil-DNA Glycosylase to prevent false positives arising from carryover contamination (i.e., contamination with amplified DNA).
Application:
The FastStart TaqMan® Probe Master is a ready-to-use, 2x concentrated master mix that contains all the reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-step qRT-PCR, in the hydrolysis probe detection format. Use the FastStart TaqMan® Probe Master with any real-time qPCR instrument other than the LightCycler® Instruments.
General description:
Other Notes:
qPCR targets
In principle, the FastStart TaqMan® Probe Master can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. However, for each target, you would need:
• to adapt your detection protocol to the reaction conditions of your real-time PCR instrument, and
• design specific PCR primers and hydrolysis probes for the target.
See the Operator′s Manual of your real-time PCR instrument for general recommendations.
Two forms available
The master mix is available in two forms – one that contains the ROX reference dye and one without ROX.
Preventing carryover contamination
This master contains dUTP, which will be incorporated into PCR products to help prevent false positives resulting from carryover contamination. In subsequent PCRs, you can add Uracil-DNA Glycosylase to degrade any uracil-containing carryover contaminants (amplification products from previous PCRs).
qRT-PCR
Combine this master mix with our Transcriptor First Strand cDNA Synthesis Kit for two-step qRT-PCR. This kit gives excellent results and works efficiently with all real-time PCR instruments.
Quality:
Function test: Each lot is tested for performance in qPCR using three templates: a GC-rich template, an AT-rich template, and a long template (approximately 440 bp).
Components:
FastStart TaqMan® Probe Master, 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, and Nucleotides (dATP, dCTP, dGTP, dUTP).
Legal Information:
LightCycler is a registered trademark of Roche