Quality Biological Buffers
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5X DNA Gel Loading Solution
Quality BiologicalDNA Gel Loading Solution, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. …
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DPBS without Ca & Mg, 1X
Quality BiologicalDPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Applications DPBS can be used to provide a buffer system to…
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1X PBS, pH 7.4
Quality BiologicalPBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …
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10X RNA Gel Buffer (10X MOPS)
Quality BiologicalMOPS Buffer is used at 1X for the electrophoresis of RNA as the running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels. MOPS buffer is also used for Northern Blots -the hybridization of total RNA or mRNA to a membrane. Liquid, 10X concentration Filtered…
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1M TRIS-HCl, pH 7.0
Quality BiologicalTris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride and provide convenient and highly purified solutions. Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its…
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Customized SDS Protein Gel Loading Solution
Quality BiologicalThis is a special formulation of loading solution for protein gel electrophoresis. Special formulation Applications SDS-PAGE Western Blotting Analysis of DNA samples with high amounts of DNA binding proteins Kinetic experiments DNA agarose gel analysis after DNA restriction digestions,…
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2X SDS Protein Gel Loading Solution
Quality BiologicalSDS Protein Gel Loading Solution, 2X is formulated at the traditional 2X stocks, for standard volume of sample to be loaded per well. Simply load the gel sample wells and visualize progress of the electrophoresis run by monitoring the location of the blue dye front. Lane Marker Sample Buffers…
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0.5M EDTA, pH 8.0
Quality BiologicalEDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and…
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1X PBS, pH 7.2
Quality BiologicalPBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …
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10X TBS, pH 7.4
Quality BiologicalTBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. DNase, RNase and Protease tested. Applications TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can…
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1X TE, pH 8.0
Quality BiologicalTE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. Filtered through a 0.22 µm…
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10X TBE
Quality BiologicalTBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. Applications EDTA is a chelator of divalent cations,…
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Trypsin 2.5%
Quality BiologicalTrypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. Without EDTA Applications Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223…
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ACK Lysing Buffer
Quality BiologicalAmmonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. Applications ACK Lysing Buffer is used to lyse red blood cells in samples, such as EDTA-treated…
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10X PBS, pH 7.4
Quality BiologicalPBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …
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10X Protein Running Buffer
Quality BiologicalProtein Running Buffer, 10X is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE), and is the running buffer for TRIS-glycine gel electrophoresis. Contains 1% SDS Filtered through a 0.22 µm…
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2M TRIS-HCl, pH 7.5
Quality BiologicalTris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride and provide convenient and highly purified solutions. Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its…
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Sodium Bicarbonate 7.5%
Quality BiologicalSodium Bicarbonate (NaHCO3) is typically used in cell culture media as a buffer. Endotoxin and Mycoplasma Tested Applications Although NaHCO3 does not have the buffering capacity of HEPES, it is occasionally preferred over HEPES because of its lower toxicity and nutritional value to cells…
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5X SDS Protein Gel Loading Solution
Quality BiologicalFormulated as 5X rather than the traditional 2X stocks, SDS Protein Gel Loading Solution 5X enables a larger volume of sample to be loaded per well. 5X concentrated protein gel loading buffer Applications Simply load the gel sample wells and visualize progress of the electrophoresis run by…
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10X RNA Gel Loading Solution
Quality BiologicalRNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989). The purpose of this reagent is to insure that the RNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. Contains two dyes, bromphenol blue…
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50X TAE Buffer
Quality BiologicalTris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications …
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Enz-Free®
Quality BiologicalL-glutamine is a cell culture supplement, and an essential amnio acid. L-glutamine is required in cell culture for cellular growth. 100X concentration Applications L-glutamine is used in cell culture as an energy source in addition to carbohydrates (i.e. glucose, sucrose, maltose, etc.).…
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Cytofluid
Quality BiologicalProprietary Sheath Fluid Prepared using water deionized by reverse osmosis 1µm sterile filtered Gamma irradiated to a minimum of 25kGy and maximum of 50kGy Applications Designed specifically for flow cytometry applications.
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2M TRIS-HCl, pH 6.8
Quality BiologicalTris HCl 2M pH 6.8 is useful with SDS-PAGE and Electrophoresis. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).Tris…
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TNE Buffer
Quality BiologicalTNE Buffer is often used as a base material to generate lysis buffers. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications More specifically, TNE Buffer is useful for: isolation of proteins from yeast cells as a lysis buffer, resuspension of pelleted virus…
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10X TAE Buffer
Quality BiologicalTris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications …
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SSC, 20X
Quality BiologicalSSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization. The concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5X. …
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DPBS with Ca & Mg, 1X
Quality BiologicalDPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Without Phenol red Applications DPBS can be used to provide a…
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10X PBS, pH 7.2
Quality BiologicalPBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture Applications , such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Applications PBS is also useful in…
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Low-EDTA 1X TE, pH 8.0
Quality BiologicalLow-EDTA 1X, pH 8.0 is molecular biology grade TE buffer used to store DNA and RNA. EDTA chelates Mg2 and other divalent metals ions – (inhibits DNAse and RNAse to suppress DNA and RNA degradation). Tris is a buffering agent to keep the solution at a defined pH – The ‘Cl’ or ‘HCl’ indicates the…
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1M Hepes Buffer, pH 7.3
Quality BiologicalHEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent at most biological pH’s and is categorized as an all- purpose buffer available for biological research. pH 7.2 – 7.6 Applications HEPES 1M is used as a buffer alternative from…
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10X TRIS Glycine Transfer Buffer (10X Transblot Buffer)
Quality BiologicalTRIS Glycine Transfer Buffer, 10X (Transblot Buffer, 10X). Applications Tris Glycine Transfer Buffer, at a 1X concentration with the addition of 20% methanol, is used for electrophoretic transfer of proteins from denaturing polyacrylamide gel to a solid membrane – i.e. Western blotting or…