Rockland Immunochemicals Inc Buffer Solutions
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FETAL BOVINE SERUM Origin: United States triple 0.1 micron filtered
Rockland Immunochemicals IncFetal Bovine Sera sourced from the United States is collected exclusively from USDA licensed abattoirs from within the United States. Raw material is processed through 0.1 µm (100nm) filters in compliance with regulatory guidelines. Animals are inspected pre-mortem and post-mortem by USDA…
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10X TTBS pH 7.5 (1.0 M Tris HCl 1.5 M Sodium Chloride 0.1% (v/v) Tween-20), 1L,…
Rockland Immunochemicals IncTBST is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. May be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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ABTS ELISA PEROXIDASE SUBSTRATE, 100mL, Liquid - clear to very pale green
Rockland Immunochemicals IncABTS ELISA PEROXIDASE SUBSTRATE is a ready to use substrate - No dilution required. Read absorbance of blue green color at 405 nm. The color formation as a function of time can be recorded at intervals or the reaction may be stopped by addition of acid after 30 mins. A 0.625 Mol L-1 Oxalic Acid…
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1.0 M Tris HCl pH 7.4, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris HCl solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This buffer consists of 1.0 M Tris HCl at pH 7.4. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or…
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5.0 M Sodium Chloride DEPC TREATED, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a 5.0M concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration.
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NORMAL RAT SERUM (NRS), 10mL, Lyophilized
Rockland Immunochemicals IncNormal Rat Serum (NRS) is a useful reagent for blocking Western Blots and ELISA wells.
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10X RIPA Lysis Buffer
Rockland Immunochemicals IncThis product is 10X concentrated stock solution. Dilute to 1X prior to use. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein immunoreactivity and…
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10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) DEPC TREATED, 1L, Liquid (sterile…
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 100 mM Tris HCl and 10 mM EDTA at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in…
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NORMAL HORSE SERUM (NHS), 10mL, Lyophilized
Rockland Immunochemicals IncHorse serum is suitable for blocking, western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
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20X SSPE Buffer (3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M…
Rockland Immunochemicals IncThis product is a 20X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA at a pH of 7.4. Meticulously prepared…
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FREUND'S COMPLETE ADJUVANT
Rockland Immunochemicals IncFreund’s adjuvant may be used to form water-in-oil emulsions with antigens for the injection of immunogens for antibody production. Proper emulsion formation is essential and should be tested by observing a drop of the emulsion on the surface of water which should 'bead and not disperse. Antigens…
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Cell Lysis Buffer, 15mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThe buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.
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NPP BUFFER (1X WORKING BUFFER), 500mL, Liquid - clear
Rockland Immunochemicals IncUtilized as the diluent buffer in ELISA experiments
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UltraPure Sterile Water (4 x 1 liter), 4L, Liquid (sterile filtered)
Rockland Immunochemicals IncSuitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
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1X RIPA Lysis Buffer 5-PACK (5 x 50 ml), 1Each, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein…
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Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate for Microwell and/or…
Rockland Immunochemicals IncPrepare FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate for use in microwell or membrane applications by mixing 1 mL of Luminol Reagent (Reagent A) with 1 mL of Reaction Buffer (Reagent B). Mix well. Protect from light. Larger or smaller volumes of the substrate can be prepared by…
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Blocking Buffer
Rockland Immunochemicals IncBlockOut® allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with IRDye® 800 and IRDye® 700DX (Licor), Cy2™,…
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DAB MEMBRANE PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncDAB Membrane Peroxidase Substrate (50x) is designed to be diluted with part number DAB-B50
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10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium Chloride, pH 7.2 prepared in highly polished…
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10X TBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X TBS Fish Gel Concentrate consists of 1.0 M Tris hydrochloride, 1.5 M sodium chloride and Fish Gelatin at pH 7.5. A…
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BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk), 500g, Lyophilized
Rockland Immunochemicals IncUse BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a…
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10% (w/v) Sodium Dodecyl Sulfate (SDS), 100mL, Liquid (sterile filtered)
Rockland Immunochemicals Inc10% SDS is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 10% (w/v) Sodium Dodecyl Sulfate (SDS). Meticulously prepared using ultra pure reagents dissolved in highly polished…
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FREUND'S INCOMPLETE ADJUVANT
Rockland Immunochemicals IncFreund’s adjuvant may be used to form water-in-oil emulsions with antigens for the injection of immunogens for antibody production. Proper emulsion formation is essential and should be tested by observing a drop of the emulsion on the surface of water which should 'bead and not disperse. Antigens…
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BCIP/NBT MEMBRANE ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale…
Rockland Immunochemicals IncBCIP/NBT Alkaline Phosphatase Membrane Substrate is a useful detection reagent for immunoblotting. The substrate is ready to use. To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color…
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TMB MEMBRANE PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB Membrane Peroxidase Substrate will produce an insoluble blue-green end product. Positive signal deposits blue-green color on membrane or surface of sample material. TMB Membrane Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB…
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10X PBST (0.2 M Potassium Phosphate 1.5 M Sodium Chloride, 0.5% (v/v) Tween-20,…
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Dilute only prior to immediate use. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium…
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NPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale…
Rockland Immunochemicals IncNPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale yellow, Expiration date is one (1) year from date of opening.
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TMB MEMBRANE PEROXIDASE SUBSTRATE. READY-TO-USE, 100mL, Liquid - clear
Rockland Immunochemicals IncTMB Membrane Peroxidase Substrate comes ready to use. No dilutions are required. TMB Membrane Peroxidase Substrate will produce an insoluble blue-green end product. Positive signal deposits blue-green color on membrane or surface of sample material. TMB Membrane Peroxidase Substrate incubation…
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Ponceau S solution, 500mL, Liquid
Rockland Immunochemicals IncStore at room temperature. The product is at working concentration, no further dilution is necessary. The limit of detection for this stain is 200 nanograms of protein after separation by electrophoresis in polyacrylamide gels and transferred to nitrocellulose or PVDF membranes. Ponceau S is not…
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1X SDS-PAGE Destain Solution (Ready-to Use), 1L, Liquid
Rockland Immunochemicals Inc1X SDS-PAGE Destain Solution is ready-to-use as a working solution and requires no further dilution.
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0.5 M EDTA pH 8.0, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncEthylenediaminetetraacetic acid is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 0.5 M Trisodium EDTA at pH 8.0. Meticulously prepared using ultra pure reagents dissolved…
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Blocking Buffer for Immunohistochemistry, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis immunohistochemistry protein blocker is specifically designed for immunohistochemical staining where detection occurs using an immunoenzymatic reaction with substrate, i.e. peroxidase (HRP) with diaminobenzidine (DAB). This product specifically inhibits non-specific staining of IHC detection…