PG-008, a fully chemically synthesized peptide, comprises a heterodimer of two cyclic peptides with selective binding affinity for Frizzled and LRP5/6. Unlike Wnt3a, PG-008 lacks lipid moieties, making it highly soluble and eliminating handling difficulties. It exhibits superior activity to Wnt3a in the agonistic activation of β-catenin pathway.
During the differentiation of iPSCs into definitive endoderm, PG-008 was compared with CHIR99021 and recombinant Wnt3a in terms of differentiation induction efficiency. The results showed that when recombinant Wnt3a (50 ng/mL: approximately 1.3 nM) was used, the efficiency reached around 60%. In contrast, when PG-008 or CHIR99021 was used, the efficiency exceeded 98%. Notably, 1 nM of PG-008 exhibited a differentiation induction efficiency equivalent to that achieved with 3 µM of CHIR99021. PG-008 demonstrated no cytotoxicity up to 10 µM, the highest concentration tested, making it a safer alternative to CHIR99021.
10 µg of PG-008 exhibits equivalent activity to 700 µg of recombinant human Wnt3a.
Provided as lyophilized powder
Purity: greater than or equal to 95% (HPLC)
MW (Da): 5099.63 (acetate)
https://peptigrowth.com/en/products/pg-008/
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