Epidermal Growth Factor (EGF), mouse natural (culture grade), a low-molecular-weight mitogenic protein that stimulates proliferation of a wide variety of cell types in vitro. (100 µg)
Formulation
EGF, mouse natural (culture grade) - Lyophilized from ammonium acetate, carrier-free; reconstitute in dH2O EGF, mouse natural (receptor grade) - Lyophilized from ammonium acetate, carrier-free; reconstitute in dH2O EGF, human recombinant - Lyophilized from acetic acid, carrier-free; reconstitute in dH2O or aqueous buffer
Preparation and Storage
EGF, mouse natural (culture grade) - Stable for at least three months at 2° to 8°C (lyophilized) or = two months at -20°C (solubilized). Do not store in frost-free freezer. Avoid multiple freeze-thaws.
EGF, mouse natural (receptor grade) - Stable for at least three months at 2° to 8°C (lyophilized) or =one month solubilized at -20°C . Do not store in frost-free freezer. Avoid multiple freeze-thaws.
EGF, human recombinant - Stable for at least three months at 2° to 8°C (lyophilized) or =three months at -20°C (solubilized). Do not store in frost-free freezer. Avoid multiple freeze-thaws.
Quality
EGF, mouse natural (culture grade)
- Purity > 95% by SDS-PAGE and > 80% by pH 9.9 PAGE
- Tested for ability to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
EGF, mouse natural (receptor grade)
- Purity > 98% by SDS-PAGE and pH 9.9 PAGE
- Tested for ability to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, and mycoplasma
EGF, human recombinant
- Purity > 98% by SDS-PAGE and reverse phase HPLC
- TShown to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
Source
EGF, mouse natural (culture grade) - Male mouse submaxillary glands
EGF, mouse natural (receptor grade) - Male mouse submaxillary glands
EGF, human recombinant - Recombinant, E. coli
Molecular Weight
EGF, mouse natural (culture grade) - 6.1 kD
EGF, mouse natural (receptor grade) - M6.1 kD
EGF, human recombinant - 6.2 kD
Purification
EGF, mouse natural (receptor grade) - Purified by BioGel® P10/DEAE chromatography
Tip
EGF, mouse natural (culture grade) - Recommended concentration - 5-100 ng/ml medium depending on cell type
EGF, mouse natural (receptor grade) - Recommended concentration - Approximately 5 ng/ml medium depending on cell type
EGF, human recombinant - Recommended concentration - 0.1-1.0 ng/ml medium depending on cell type