Click's Medium is a modification of Eagle's Essential Medium with Hanks' Salts (HMEM) and contains higher concentrations of essential amino acids, in addition to non-essential amino acids, sodium pyruvate, and nucleic acid precursors.
With:
- 1350 mg/L Sodium Bicarbonate
Without:
- Mercaptoethanol
- L-Glutamine - add 20mL/L (200mM)
500 mL, 24 month shelf life