The APIS DPYD qPCR Kit is designed for fast and efficient detection of seven DPYD alleles. Each reaction identifies both wildtype (WT) and variant alleles, directly from blood or extracted genomic DNA (gDNA). The kit’s rapid turnaround time, cost-effectiveness, and easy integration into existing qPCR workflows make it a reliable and accessible option for a variety of research applications.
Advantages of the APIS DPYD qPCR Kit
- Wide target coverage – detects seven DPYD variants
- Direct Workflow – compatible with both 1% EDTA Whole Blood and gDNA
- Reliable HapB3 detection – targets key functional HapB3 variant
- Accessible – compatible with most qPCR instruments
The analytical sensitivity of the kit was evaluated by testing homozygous (wildtype) and heterozygous samples and defined as the minimum amount of DNA necessary to correctly genotype 95% of the samples. The APIS DPYD qPCR Kit can detect seven DPYD variants in as little as 0.11ng gDNA.
APIS DPYD qPCR Kit shows excellent concordance with alternative research genotyping methods. gDNA extracted from 29 specimens with known DPYD variant status (characterised via NGS, microarrays or qPCR) and one synthetic DNA sample were assessed with the APIS DPYD qPCR Kit.
The kit has undergone testing as part of APIS’ unique RUO assay development pipeline, including both in silico and wet laboratory testing. As a result, researchers can depend on the kit for consistent and high-quality detection of DPYD variants. A positive and negative control (PC and NTC), which monitor assay set up and assay performance, are also supplied with the kit.
The APIS DPYD qPCR Kit is available as a Research Use Only (RUO) product. Not applicable for use in diagnostic procedures. Kit size: 24 samples plus controls, includes: 7x Primer Probe mixes, 2x Enzyme Mixes, Positive Control, No Template Control (NTC).