It is a rapid, inexpensive, and versatile methodthat is applicable to the purification of many proteins and to demonstrate principles of bio affinitychromatography. Dye chromatography use mimics of natural protein ligands to act as a pseudo-affinity chromatography. Reactive dyes are immobilized to…

Cu-immobilized metal affinity chromatography (IMAC) columns consist of Cu-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Cu-IMAC Columns separate proteins via their differences in affinity to Cu2+ metal ions. Ideal for purification of recombinant…

Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Provided as pre-packed,…

Zerobind™ affinity columns are designed to remove non-specific backgrounds from various polyclonal antibodies generally used for western blotting. Passing the diluted Antibody solution through Zerobind™ column can reduce non-specific binding while retaining activity against your…

Macro-spin columns have a packing volume of 500 μl suitable for centrifugation of 50-150 μl sample volume. Prepacked with Sephadex G-50 Collect DNA with small MW (10-20 bases) Suitable for use with Swing-out Rotor Centrifuge No High Speed needed avg, speed needed is 1000-2000…

An affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP).  Amylose is linked to Separopore® (Agarose) 4B-CL. The resin may be generated up to five times.  Store used resin in column buffer containing 0.02% sodium azide. Quantitative Analysis: Protein…

Ni-immobilized metal affinity chromatography (IMAC) columns consist of Ni-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Ni-IMAC Columns separate proteins via their differences in affinity to nickel metal ions. Ideal for purification of recombinant…

Erythrina cristagalli (coral tree) lectin is a 54,000 dalton glycoprotein consisting of two different subunits of approximately 28,000 and 26,000 daltons. It has a specificity toward galactose residues and appears to have the highest binding activity toward galactosyl ( b -1,4)…

Zn-immobilized metal affinity chromatography (IMAC) columns consist of Zn-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Zn-IMAC Columns separate proteins via their differences in affinity to Zn2+ metal ions. Ideal for purification of recombinant…

bio-WORLD's immunoaffinity columns will remove many of the abundant proteins in serum that interfere with proteomic analysis. These include albumin, transferrin, haptoglobin, alpha-1-antitrypsin, and many of the immunoglobins. By removing these proteins, the remaining serum can be concentrated,…

Benzamidine Separopore®6B is p-aminobenzamidine covalently attached to Separopore® 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is an inhibitor of trypsin-like serine protease. Trypsin and trypsin-like serine proteases bind to Benzamidine Separopore® 6B and can thus be used for…

Peanut agglutinin (PNA, Peanut Agglutinin) is a 110,000 molecular weight lectin composed of four identical subunits of approximately 27,000 daltons each. PNA binds preferentially to a commonly occurring structure, galactosyl (b-1,3) N-acetylgalactosamine. This carbohydrate sequence (called the…

Jacalin, α D-galactose-specific lectin from jackfruit, interacts with human IgA and one or two other serum proteins. Jacalin coupled Separpore ® is used for the purification of glycoproteins containing α-D-galactose. bio-World provides Jacalin-Separpore ® as a suspension…