Ni-immobilized metal affinity chromatography (IMAC) columns consist of Ni-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Ni-IMAC Columns separate proteins via their differences in affinity to nickel metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.
- Imminodiacetate immobilized matrix charged with Ni metal ions.
- Chelating group coupling method: epoxy activationv
- Matrix: cross-linked Separopore® 6B
- Particle size (range): 50-170µm v
- Metal ion capacity: > 20µmol Ni2+/ml gel
- Spacer arm: 14 atomv vpH stability: 3-12
- Binding Capacity: Approx. 40 mg His-tagged protein/ml medium
- Supplied as suspension in 20% ethanol.