It is a rapid, inexpensive, and versatile methodthat is applicable to the purification of many proteins and to demonstrate principles of bio affinitychromatography. Dye chromatography use mimics of natural protein ligands to act as a pseudo-affinity chromatography. Reactive dyes are immobilized to a solid support, Separopore®(Agarose) matrix. The dyes act as a competitive inhibitor for a protein’s normal ligand.
Several reactive textile dyes have been found to bind to proteins especially those with affinities to various nucleotides. Immobilized dyes have been found to bind from 5 to 60% of the proteins in various crude cell extracts. The affinity for reactive dyes to proteins may be due to substrate/cofactor similarities, as well as hydrophobic and ion exchange properties.he affinity or specificity of a dye for a particular nucleotide binding site is not very well predicted, the most effective method for the determination of a specific protein's binding capacity is a screening procedure with several types of immobilized reactive dyes.
bio-WORLD's bioSPECTRUM™ Dye affinity Set includes a trial size, six-pack prepacked column of 2.5 ml each:
- Reactive Red-120 Separopore® 4B-CL
- Blue (Cibacron Blue F3G-A) Separopore® 4B-CL
- Reactive blue-4 Separopore® 4B-CL
- Reactive Yellow-86 Separopore® 4B-CL
- Reactive Green-19 Separopore® 4B-CL
- Reactive Brown-10 Separopore® 4B-CL