Terrific Broth is an enriched medium, which helps the recombinant strains of E. coli maintain an extended growth phase. The large amount of tryptone and yeast extract in the medium allows the bacteria to produce a higher plasmid yield. Autoclave sterilized Ready to use Applications …

Molecular Biology Water is prepared by reverse osmosis, passed through fine carbon, deionized through two resin beds, and serially filtered through 0.1 µm positively charged membranes, to yield a product with an endotoxin level of less than 0.03 EU/mL. DNase, RNase and protease tested Endotoxin…

Cell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without Phenol Red With Glucose and L-glutamine Applications EMEM (Eagle’s Minimum Essential Medium) has…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With: High Glucose Without: HEPES MEM Spinner was developed when calcium component was removed from the MEM medium, and resulted in a medium that supports the…

Alpha MEM is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Recommendation This product is made with Earle’s salts. MEM a contains no proteins, lipids, or…

Applications Standard, unstabilized solutions of L-glutamine spontaneously break down over time to release cytotoxic amines. Glutamine XL is a stabilized solution that prevents degradation and cytotoxicity, with the benefit of a long shelf life. The incorporated L-alanine protects the unstable…

E199 or Medium 199 with Earle's Salt is one of the best known media in the history of cell culture. Morgan, Morton and Parker's Medium 199 was the first really successful attempt to develop a totally defined medium. Medium 199 was originally developed for nutritional studies of chick embryo…

USDA FBS is used as a supplement in classical cell culture media. USDA FBS both nourishes and protects the cells in a way that almost nothing else has been found to do. USDA Approved – Premium Grade Bovine serum stimulates the cells to grow and multiply and helps to keep the cells…

LB Agar 1.5% Lennox provides all nutritional requirements of these organisms. LB Agar 1.5% Lennox contains half the sodium chloride level of the Miller formulation of LB Agar. Just autoclave to melt and pour. Applications LB Agar 1.5% Lennox is nutritionally rich. It was developed by…

DMEM is one of the most widely used modifications of Basal Medium Eagle (BME). DMEM was originally developed as a richer version of BME; with 4 times higher concentrations of amino acids, vitamins and some additional components than BME. DMEM is commonly used for the culture of normal and…

Dr. Williams and his coworkers’ studies on hepatocytes in culture led to the development of Williams’ Medium E. Without L-glutamine Applications This rich medium was used to culture the hepatocytes derived from an enrichment procedure also developed by Dr. Williams and his colleagues in…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…

SOC medium is a ready-to-use solution, useful in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E. coli. Without magnesium chloride, needs to be added before use. Autoclave sterilized Applications SOC is a microbial growth medium used for…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate Without HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…

Compared to other basal media, Ham’s F-12 contains a wider variety of components, and no proteins or growth factors. Without hypoxanthine and L-glutamine Ham’s F-12 uses a sodium bicarbonate buffer system (1.176 g/L); therefore requiring a 5–10% CO2 environment to maintain physiological pH. …

Synthetic Dextrose (SD) Medium is a defined minimal yeast medium. Applications SD is a synthetic minimal medium containing salts, trace elements, vitamins, a nitrogen source (Bacto-yeast nitrogen base without amino acids), and glucose.

L-glutamine is a cell culture supplement, and is required in cell culture for cellular growth. L-glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, gluthione, L-glutamate and other amino acids, as well as in protein synthesis and glucose production.…

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators…

Non Fat Dry Milk Can be used for making blocking solution

LB Broth, Lennox is a ready-to-use microbial growth medium used for the cultivation of E. coli. Low (5 g/L) salt formulation Autoclave sterilized Applications LB Broth is a low-salt formulation in comparison to its counterpart LB Broth, Bertani Miller. Lennox is also nutrient rich…

Sodium pyruvate is commonly utilized as an additive in culture media and broth as a carbon source, similar to glucose, as pyruvate is the end product of the glycolysis pathway. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement…

YPD/YPED Plates are used for the growth of Saccharomyces cerevisiae (yeast). Applications A solid medium for the propagation of yeast.

This modification of Dulbecco's MEM was developed by Dr. Iscove and his coworkers to assist in their studies of hematopoietic stem cells. They modified Dulbecco's MEM by the addition of HEPES buffer, selenium, additional amino acids, vitamins and sodium pyruvate, and potassium nitrate…

Applications The FBS in the Cryopreservation Medium improves the post-freezing recovery and survival after cryopreservation, while DMSO preserves the cells during freeze. Cryopreservation Medium with Fetal Bovine Serum (FBS) and Calf Serum is a complete cryopreservation medium with 10% DMSO.

McCoy’s 5A Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. With L-glutamine and Phenol Red McCoy’s 5A Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a…

YES Agar Plates are for use of propagation and maintenance of yeasts for use in molecular biology Applications YES Agar Plates are for the growth of yeast.

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With High Glucose Without L-Glutamine Without Phenol red Without HEPES Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells,…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With: High Glucose Without: HEPES Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…