• PRODUCT AVAILABILITY: Did you know you can view a product's availability right on the product page? Simply enter the quantity you want to purchase and the current availability will appear below the item.

  • Product C837J98 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

Quality Biological

RPMI-1640 with 25mM Hepes Buffer

Not yet rated

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS).

With 2.0 g/L sodium bicarbonate
With HEPES buffer

Applications
RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures.

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). Although, RPMI-1640 has been shown to support the serum-free growth of a number of cells.

Common applications of RPMI-1640 include: fusion protocols and growth of hybrid cells, culture of normal human leukocytes in monolayer and suspension, and support of HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinoma growth (to name a few).

Storage Conditions: 2°C to 8°C

Shipping Condition: ambient temperature

Sterile Filtered

pH: 7.0 ± 0.2

Osmolality: 300 ± 5% mOsm/Kg H2O

Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health

Harmonization Code: 3821.00.0000

ECCN: EAR99

Max Shelf Life: 24 months

Please Enter Your Order Info

Filter by:

  • Clear Filters
Product Detail
Thomas No.
C837J98
Mfr. No.
112-143-101
Description
RPMI-1640 w/ 25mM Hepes Buffer, 500mL
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product C837J98 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

Write A Review