Formulated as 5X rather than the traditional 2X stocks, SDS Protein Gel Loading Solution 5X enables a larger volume of sample to be loaded per well. 5X concentrated protein gel loading buffer Applications Simply load the gel sample wells and visualize progress of the electrophoresis run by…

M9 CA Media contains the essentials for bacterial species to grow. Glucose as a carbon source and casamino acids as a nitrogen source. Important components of M9 CA medium are the carbon source from glucose, a nitrogen source from ammonia chloride, and electrolytes from sodium, chloride,…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We offer a variety of DMEM modifications…

RNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989). The purpose of this reagent is to insure that the RNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. Contains two dyes, bromphenol blue…

Nuclease and Endotoxin-Free, Sterile DEPC-treated water is certified nuclease-free and supplied in four bottles containing 1 L each to ensure sterility and inactivation of DEPC. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity, protease. …

SOC medium is a ready-to-use solution, useful in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E. coli. Without magnesium chloride, needs to be added before use. Autoclave sterilized Applications SOC is a microbial growth medium used for…

Nutritionally rich media designed for growth of pure cultures of recombinant strains. 10 g/L of salt formulation Autoclave sterilized LB Agar, Miller and LB Broth, Miller are based on LB Medium as described by Miller for the growth and maintenance of E. coli strains used in molecular…

SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization. The concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5X. …

Terrific Broth is an enriched medium, which helps the recombinant strains of E. coli maintain an extended growth phase. The large amount of tryptone and yeast extract in the medium allows the bacteria to produce a higher plasmid yield. Autoclave sterilized Ready to use Applications …

Synthetic Dextrose (SD) Medium is a defined minimal yeast medium. Applications SD is a synthetic minimal medium containing salts, trace elements, vitamins, a nitrogen source (Bacto-yeast nitrogen base without amino acids), and glucose.

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. Applications ACK Lysing Buffer is used to lyse red blood cells in samples, such as EDTA-treated…

This is a special formulation of loading solution for protein gel electrophoresis. Special formulation Applications SDS-PAGE Western Blotting Analysis of DNA samples with high amounts of DNA binding proteins Kinetic experiments DNA agarose gel analysis after DNA restriction digestions,…

Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Filtered through a 0.22 µm membrane DNase,…

Amphotericin B, Penicilllin, Stretomycin Mix is useful for reducing the chance of microbial contamination. Convenient mixture of the antibiotics and antifungal agent in one bottle. 100X concentration, just dilute 1:100 in the culture medium Recommended for use in cell culture applications at…

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. Ready-to-use solution Applications Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis and…

Super Broth is used for the mass cultivation of Escherichia coli for high yield protein and plasmid DNA production. Has 220% more peptone and 300% more yeast extract than LB Autoclave sterilized Applications Super Broth is the best commonly used E. Coli growth medium for plasmid…

SDS Protein Gel Loading Solution, 2X is formulated at the traditional 2X stocks, for standard volume of sample to be loaded per well. Simply load the gel sample wells and visualize progress of the electrophoresis run by monitoring the location of the blue dye front. Lane Marker Sample Buffers…

Tris HCl 2M pH 6.8 is useful with SDS-PAGE and Electrophoresis. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).Tris…

This modification of Dulbecco's MEM was developed by Dr. Iscove and his coworkers to assist in their studies of hematopoietic stem cells. They modified Dulbecco's MEM by the addition of HEPES buffer, selenium, additional amino acids, vitamins and sodium pyruvate, and potassium nitrate…

Sodium pyruvate is commonly utilized as an additive in culture media and broth as a carbon source, similar to glucose, as pyruvate is the end product of the glycolysis pathway. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement…

RPMI was developed to support lymphoblastoid cells in culture, RPMI-1640 has now been successfully used for the culture of a wide range of anchorage independent cells. Although intended for use with serum supplementation, RPMI-1640 has been shown to support the serum-free growth of a number of…

MOPS Buffer is used at 1X for the electrophoresis of RNA as the running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels. MOPS buffer is also used for Northern Blots -the hybridization of total RNA or mRNA to a membrane. Liquid, 10X concentration Filtered…

Sodium Chloride, 0.85% is a saline solution at 0.85% prepared with water used for cell culture. Made with cell culture grade water The sodium chloride is a source of chlorine and sodium necessary to the production of components like sodium carbonate, sodium hydroxide or chlorate ions. …

YPD/YPED Plates are used for the growth of Saccharomyces cerevisiae (yeast). Applications A solid medium for the propagation of yeast.

DNA Gel Loading Solution, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. …

Cell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend…

Designed for cell culture without CO2. Without Sodium Bicarbonate With Phenol Red Without L-glutamine Applications L-15 has been used for the culture of HEp-2 monkey kidney and primary explants of embryonic and adult human tissue, and a variety of other cells. Many viruses have also been…

Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Reduce nonspecific binding Applications Quality Biological’s Blocking Solution is prepared from non-fat dried milk in PBS containing sodium azide 0.02% (w/v) as a…

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture Applications , such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Applications PBS is also useful in…

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Without Phenol red Applications DPBS can be used to provide a…

Formulated as 5X rather than the traditional 2X stocks, SDS Protein Gel Loading Solution 5X enables a larger volume of sample to be loaded per well. 5X concentrated protein gel loading buffer Applications Simply load the gel sample wells and visualize progress of the electrophoresis run by…

Sodium Bicarbonate (NaHCO3) is typically used in cell culture media as a buffer. Endotoxin and Mycoplasma Tested Applications Although NaHCO3 does not have the buffering capacity of HEPES, it is occasionally preferred over HEPES because of its lower toxicity and nutritional value to cells…